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Zitierungen und Referenzen (25)
Invitrogen™
Gateway™ pDEST™17 Vector
Um all Ihren Expressionsansprüche zu entsprechen, bietet Invitrogen modernste Gateway™-Zielvektoren für die Expression in E. coli-, Insekten-, Hefe- oder SäugetierzellenWeitere Informationen
| Katalognummer | Menge |
|---|---|
| 11803012 | 6μ g |
Katalognummer 11803012
Preis (EUR)
1.098,00
Each
-
Menge:
6μ g
Preis (EUR)
1.098,00
Each
Um all Ihren Expressionsansprüche zu entsprechen, bietet Invitrogen modernste Gateway™-Zielvektoren für die Expression in E. coli-, Insekten-, Hefe- oder Säugetierzellen sowie für die Produktion von nativen Proteinen bzw. von Fusionsproteinen am N- bzw. C-Terminal. Alle Gateway™-Zielvektoren verfügen über attR-Stellen für die Rekombination mit jedem von attL-flankierten Fragment, unabhängig davon, ob es sich um einen Entry-Klon oder einen Ultimate™ RF-Klon handelt. In der folgenden Tabelle sind die zahlreichen verfügbaren Zielvektoren aufgeführt.
Zusätzliche Materialien erforderlich, separat erhältlich:
Gateway™ Entry-Klon, Gateway™ LR Clonase™ Enzymgemisch und Reaktionspuffer.
Zusätzliche Materialien erforderlich, separat erhältlich:
Gateway™ Entry-Klon, Gateway™ LR Clonase™ Enzymgemisch und Reaktionspuffer.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Bakterielle AntibiotikaresistenzAmpicillin (AMPR)
Konstitutives oder induktives SystemInduzierbar
ProdukttypExpressionsvektor
Menge6μ g
Selektionsmittel (eukaryotisch)Keine
VektorpDEST, Gateway T7 Vektoren
KlonierungsmethodeGateway™
ProduktlinieGateway™
PromoterT7
ProteinmarkierungHis-Tag (6x)
Unit SizeEach
Inhalt und Lagerung
pDEST™ 17 Vektor wird in TE-Puffer (pH 8,0) bei 150 ng/µl geliefert. Bei -20 °C lagern. Bei ordnungsgemäßer Lagerung garantiert 6 Monate lang haltbar.
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Häufig gestellte Fragen (FAQ)
In the single-step protocol for the BP/LR Clonase reaction, we would not recommend substituting the BP Clonase II/LR Clonase II enzymes with BP Clonase /LR Clonase enzymes as this would result in very low recombination efficiency.
Yes, we have come up with a single-step protocol for BP/LR Clonase reaction (http://www.thermofisher.com/us/en/home/life-science/cloning/gateway-cloning.html#1), where DNA fragments can be cloned into Destination vectors in a single step reaction, allowing you to save time and money.
We would recommend performing a BP reaction with a Donor vector in order to obtain an entry clone. This entry clone can then be used in an LR reaction with the Destination vector to obtain the new expression clone.
We do not offer the 5X LR Clonase buffer and 5X BP Clonase buffer as standalone products. They are available as part of the enzyme kits.
We do not offer any Gateway vectors for expression in plants.
Zitierungen und Referenzen (25)
Search citations by name, author, journal title or abstract text
Zitierungen und Referenzen
Abstract
Munc13-4 is a GTP-Rab27-binding protein regulating dense core granule secretion in platelets.
Journal:J Biol Chem
PubMed ID:14699162
'Platelets store self-agonists such as ADP and serotonin in dense core granules. Although exocytosis of these granules is crucial for hemostasis and thrombosis, the underlying mechanism is not fully understood. Here, we show that incubation of permeabilized platelets with unprenylated active mutant Rab27A-Q78L, wild type Rab27A, and Rab27B inhibited the
Crystal structure of Thermotoga maritima alpha-L-fucosidase. Insights into the catalytic mechanism and the molecular basis for fucosidosis.
Journal:J Biol Chem
PubMed ID:14715651
'Fucosylated glycoconjugates are involved in numerous biological events, and alpha-l-fucosidases, the enzymes responsible for their processing, are therefore of crucial importance. Deficiency in alpha-l-fucosidase activity is associated with fucosidosis, a lysosomal storage disorder characterized by rapid neurodegeneration, resulting in severe mental and motor deterioration. To gain insight into alpha-l-fucosidase function
The oncoprotein Tax binds the SRC-1-interacting domain of CBP/p300 to mediate transcriptional activation.
Journal:Mol Cell Biol
PubMed ID:11463834
'Oncogenesis associated with human T-cell leukemia virus (HTLV) infection is directly linked to the virally encoded transcription factor Tax. To activate HTLV-1 transcription Tax interacts with the cellular protein CREB and the pleiotropic coactivators CBP and p300. While extensively studied, the molecular mechanisms of Tax transcription function and coactivator utilization
Human DNA polymerase N (POLN) is a low fidelity enzyme capable of error-free bypass of 5S-thymine glycol.
Journal:J Biol Chem
PubMed ID:16787914
'Human DNA polymerase N (POLN or pol nu) is the most recently discovered nuclear DNA polymerase in the human genome. It is an A-family DNA polymerase related to Escherichia coli pol I, human POLQ, and Drosophila Mus308. We report the first purification of the recombinant enzyme and examination of its
Dual-tagging system for the affinity purification of mammalian protein complexes.
Journal:Biotechniques
PubMed ID:17907572
'Although affinity purification coupled with mass spectrometry (MS) provides a powerful tool to study protein-protein interactions, this strategy has encountered numerous difficulties when adapted to mammalian cells. Here we describe a Gateway-compatible dual-tag affinity purification system that integrates regulatable expression, tetracysteine motifs, and various combinations ofaffinity tags to facilitate the
25 total citations