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Zitierungen und Referenzen (68)
Gibco™
Penicillin-Streptomycin (10.000 U/ml)
Diese Lösung enthält 10.000 Einheiten/ml Penicillin und 10.000 μg/ml Streptomycin. Die Antibiotika Penicillin und Streptomycin verhindern mit ihrer kombinierten WirkungWeitere Informationen
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| Katalognummer | Menge |
|---|---|
| 15140122 auch als 15140-122 bezeichnet | 100 mL |
| 15140148 auch als 15140-148 bezeichnet | 20 mL |
| 15140163 auch als 15140-163 bezeichnet | 20 x 100 mL |
Katalognummer 15140122
auch als 15140-122 bezeichnet
Preis (EUR)
26,65
Online Exclusive
27,84Ersparnis 1,19 (4%)
Each
-
Menge:
100 mL
Preis (EUR)
26,65
Online Exclusive
27,84Ersparnis 1,19 (4%)
Each
Diese Lösung enthält 10.000 Einheiten/ml Penicillin und 10.000 μg/ml Streptomycin. Die Antibiotika Penicillin und Streptomycin verhindern mit ihrer kombinierten Wirkung gegen grampositive und gramnegative Bakterien eine bakterielle Kontamination von Zellkulturen. Penicillin, das ursprünglich aus dem Pilz Penicillium gewonnen wurde, wirkt direkt, indem es den Neuaufbau der bakteriellen Zellwand stört, sowie indirekt, indem es die Freisetzung von Enzymen auslöst, die die Zellwand weiter verändern. Streptomycin wurde ursprünglich aus Streptomyces griseus isoliert. Es wirkt, indem es sich an die 30S-Untereinheit des bakteriellen Ribosoms anlagert, was zu einer Hemmung der Proteinsynthese und anschließendem Absterben sensitiver Bakterien führt.
Wir bieten eine breite Palette an Antibiotika und Antimykotika in Pulver- und flüssiger Form an. Erfahren Sie mehr über die folgenden Produkttypen:
• Zellkultur Antibiotika-Auswahl
• Selektive Antibiotika (einschließlich empfohlener Arbeitskonzentrationen)
Erfahren Sie mehr über die Verwendung von Antibiotika und Antimykotika in der Zellkultur und lesen Sie Richtlinien für die Dekontamination von Kulturen.
Wir bieten eine breite Palette an Antibiotika und Antimykotika in Pulver- und flüssiger Form an. Erfahren Sie mehr über die folgenden Produkttypen:
• Zellkultur Antibiotika-Auswahl
• Selektive Antibiotika (einschließlich empfohlener Arbeitskonzentrationen)
Erfahren Sie mehr über die Verwendung von Antibiotika und Antimykotika in der Zellkultur und lesen Sie Richtlinien für die Dekontamination von Kulturen.
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Konzentration100X
Zur Verwendung mit (Anwendung)Zur Prävention von Zellkulturkontaminationen
Menge100 mL
Haltbarkeit12 Monate
VersandbedingungTrockeneis
FormFlüssig
ProdukttypAntibiotikum
SterilitätSteril gefiltert
Sterilization MethodSterile-filtered
Unit SizeEach
Inhalt und Lagerung
Lagerbedingungen: -5 bis -20 °C
Versandbedingungen: Trockeneis
Haltbarkeit: 12 Monate ab Herstellungsdatum
Versandbedingungen: Trockeneis
Haltbarkeit: 12 Monate ab Herstellungsdatum
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Zertifikate
Nach Chargennummer oder Teil-Chargennummer suchen
Chargen-Nr.Certificate TypeDateCatalog Number(s)
253952Certificate of Analysis06. Juni 202515140148, 15140122
253664Certificate of Analysis14. Mai 202515140148, 15140122
253663Certificate of Analysis03. Mai 202515140148, 15140122
251306Certificate of Analysis03. Mai 202515140148, 15140122
250552Certificate of Analysis12. Apr. 202515140148, 15140122
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Sicherheitsdatenblätter
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Häufig gestellte Fragen (FAQ)
Yes, this is normal and will not affect the potency or application of the product. This solution is typically colorless. However, it can have a pink to yellow color tint. The coloring is a carry-over from the manufacturing process of Streptomycin - the genus that Steptomycin is isolated from (Actinomycetes Streptomyces griseus) is responsible for a wide variety of pigments.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.
1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.
The following is a suggested procedure for determining toxicity levels and decontaminating cultures:
1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Zitierungen und Referenzen (68)
Search citations by name, author, journal title or abstract text
Zitierungen und Referenzen
Abstract
Identification of a novel redox-sensitive gene, Id3, which mediates angiotensin II-induced cell growth.
Journal:Circulation
PubMed ID:12021231
BACKGROUND: Reactive oxygen species, such as superoxide (O(2)(-)), are involved in the abnormal growth of various cell types. Angiotensin II (Ang II) is one of the most potent inducers of oxidative stress in the vasculature. The molecular events involved in Ang II-induced proliferation of vascular smooth muscle cells (VSMCs) are
Functional interaction of caveolin-1 with Bruton's tyrosine kinase and Bmx.
Journal:J Biol Chem
PubMed ID:11751885
'Bruton''s tyrosine kinase (Btk), a member of the Tec family of protein-tyrosine kinases, has been shown to be crucial for B cell development, differentiation, and signaling. Mutations in the Btk gene lead to X-linked agammaglobulinemia in humans and X-linked immunodeficiency in mice. Using a co-transfection approach, we present evidence here
Involvement of c-Src Tyrosine Kinase Upstream of Class I Phosphatidylinositol (PI) 3-Kinases in Salmonella Enteritidis Rck Protein-mediated Invasion.
Journal:J Biol Chem
PubMed ID:22810232
'The Salmonella outer membrane protein Rck mediates a Zipper entry mechanism controlled by tyrosine phosphorylation and class I phosphatidylinositol 3-kinase (PI 3-kinase). However, the underlying mechanism leading to this signaling cascade remains unclear. The present study showed that using Rck-coated beads or Rck-overexpressing Escherichia coli, Rck-mediated actin polymerization and invasion
Repression of activator protein-1-mediated transcriptional activation by the Notch-1 intracellular domain.
Journal:J Biol Chem
PubMed ID:11739397
'Developmental decisions that control cell fate are commonly regulated by the Notch signaling pathway. Activation of transmembrane Notch receptors results in proteolytic liberation of the intracellular domain of Notch, which translocates into the nucleus, binds a repressor (C promoter binding factor 1/RBP-Jkappa, Su(H), and Lag-1 (CSL)), and induces target genes.
A differential role for the mitogen-activated protein kinases in lipopolysaccharide signaling: the MEK/ERK pathway is not essential for nitric oxide and interleukin 1beta production.
Journal:J Biol Chem
PubMed ID:11786532
'Endotoxin (lipopolysaccharide, LPS) is a component of the outer membrane of Gram-negative bacteria and promotes the activation of macrophages and microglia. Although these cells are highly LPS-responsive, they serve unique tissue-specific functions and exhibit different LPS sensitivities. Accordingly, it was of interest to evaluate whether these biological differences reside in
68 total citations