RPMI 1640 Medium, Pulver, HEPES

Gibco™

RPMI 1640 Medium, Pulver, HEPES

Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as aWeitere Informationen

Katalognummer	Menge
23400062	10 l

Katalognummer 23400062

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107,00

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Menge:

10 l

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Preis (EUR)

107,00

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Roswell Park Memorial Institute (RPMI) 1640 medium was originally developed to culture human leukemic cells in suspension and as a monolayer. RPMI 1640 has since been found suitable for a variety of mammalian cells including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes and carcinomas. Life Technologies offers a variety of Gibco™ RPMI 1640 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This RPMI is modified as follows:

With	Without
• L-glutamine	• Sodium Bicarbonate
• Phenol Red
• HEPES

The complete formulation is available.

Gibco™ RPMI 1640 is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 contains biotin, vitamin B₁₂ and PABA which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations.

Product Intended Use
For in vitro diagnostic use. CAUTION: Not for human or animal therapeutic use. Uses other than the intended use may be a violation of local law. Customers using Gibco™ RPMI 1640 in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

RPMI 1640 contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). RPMI 1640 uses a sodium bicarbonate buffer system (2.0 g/L) and therefore requires a 5-10% CO₂ environment to maintain physiological pH.

Powder forms of Gibco™ cell culture medium require sodium bicarbonate supplementation, pH adjustment, and filtration at the time of preparation (see protocol for details).

Nur für Forschungszwecke.Nicht zur Verwendung bei diagnostischen Verfahren.Nicht geeignet für therapeutische Zwecke bei Menschen und Tieren.Zweckentfremdete Verwendung kann eine Verletzung lokaler gesetzlicher Bestimmungen darstellen.Kunden, die Gibco RPMI 1640 in einem Herstellungsprozess verwenden und dafür bei der FDA eine Lizenz beantragt haben, können bei Life Technologies eine Vollmacht als Referenz für unser Typ II Drug Master File (DMF) erhalten.Zur Verwendung in der In-vitro-Diagnostik.

Specifications

ZelllinieHeLa, Jurkat, MCF-7, PC-12, PBMC, Astrozyten und Karzinome

ZelltypLeukämische Zellen

Fertigungsqualität, HerstellungsqualitätcGMP-compliant under the ISO 13485 standard

ProduktlinieGibco™

ProdukttypRPMI 1640 Medium (Roswell Park Memorial Institute 1640 Medium)

Menge10 l

Haltbarkeit36 Monate ab Herstellungsdatum

VersandbedingungRaumtemperatur

KlassifikationOhne Stoffe tierischen Ursprungs

FormPulver

Serum LevelStandard-Serumzugabe

SterilitätSteril gefiltert

Mit AdditivenGlutamin, HEPES, Phenolrot

Ohne AdditiveOhne Natriumpyruvat, Kein Natriumhydrogencarbonat

Unit SizeEach

Inhalt und Lagerung

Lagerbedingungen:2–8 °C
Versandbedingungen:Haltbarkeit bei
Raumtemperatur:36 Monate ab Herstellungsdatum

Explore all Gibco RPMI 1640 for reproducible cell culture results ›

Medienformulierungen

23400 - RPMI 1640, powder, HEPES

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Häufig gestellte Fragen (FAQ)

How light sensitive is RPMI 1640 media? Should I also be protecting it from LED light?

While we know that different wavelengths of light are worse than others for exposure, we would recommend as a best practice to protect the medium from all forms of light exposure including LEDs, as much as possible to ensure optimal performance, as several components within the medium are light sensitive, such as vitamins.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the density (g/L) for RPMI 1640 Medium?

We have specific gravity information for RPMI 1640 Medium: 1.006 kg/L. In this case, the specific gravity is the same as density as the solvent is water.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the shelf life of my powdered media once I reconstitute it?

Expiration date of most Gibco reconstituted dry format media (AGT or DPM) has not been established; end users should assess performance and stability of this reconstituted media in a system that is relevant to their process. A dry format product (either DPM or AGT) will age upon storage and while we may not be able to detect which component(s) degrade since we can't measure every component, that doesn't mean that the reconstituted liquid will have the same stability as a fresh liquid made by direct weigh methods.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

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