Zeba™ Spin Desalting Plates, 7K MWCO

You may remove excess solvent and smaller moieties by centrifugation through a microconcentrator. This will concentrate your protein sample.

(1) Choose microconcentrator tube (available from a variety of commercial suppliers) with a protein cutoff smaller than the molecular weight of the protein in the sample. Check our Pierce Protein Concentrators PES.

(2) Add 1 µL of 20% w/v SDS to a dry microconcentrator tube (if sample does not already contain SDS).

(3) Slowly add sample (a few microliters at a time) to membrane until membrane is completely wet. Centrifuge to near (but not complete) dryness.

(4) If intention is to desalt sample or buffer exchange: Add ~50 µL water to microconcentrator and spin until nearly dry. Repeat buffer exchange. Sample will remain on membrane. Check our Zeba desalting proteins.

(5) Using a new collection tube, invert membrane and spin at low speed (1000 x g) to elute protein from membrane. Add 2X SDS-Sample Buffer containing 10 mM DTT to membrane: vortex, invert and spin. Final volume should be ~20 µL.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Yes, Zeba Desalting columns, plates, and cartridges can be autoclaved at 115 degrees C for 30 min.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

Zeba Desalting columns, plates, and cartridges are compatible with a range of reagents and conditions. They have been confirmed to be compatible with the following:
- pH range of 3-10
- Mild oxidants/reductants
- Chaotropes (stable in 8 M urea and 6 M guanidine HCl)
- Salts
- Alcohols up to 20%
- Organic solvents such as dimethyl sulfoxide (DMSO) and dimethylformamide (DMF) (we recommend using a step-gradient of increasing concentration of the organic solvent during column equilibration)

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

We recommend storing Zeba Spin Desalting Columns, Plates, and Cartridges at 4 degrees C.

Find additional tips, troubleshooting help, and resources within our Protein Dialysis, Desalting, and Concentration Support Center.

We recommend using Zeba Spin Desalting Columns, Plates, and Cartridges (Cat. No. 89882, 89883) to remove sodium azide from the Alix Monoclonal Antibody (3A9) storage solution.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.