Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Katalognummer | Menge |
---|---|
A1084001 | 1 x 106 Zellen |
A1084002 | 4 x 106 |
Rat cortical neurons (A1084001) transfected with CellLight??? Golgi-RFP, BacMam 2.0 (C10593). At 48 hours cells were fixed and stained with anti-Tau (MN1000), followed by Goat anti-Mouse Alexa Fluor Plus 488 (A32723) and NucBlue??? Fixed Cell ReadyProbes??? Reagent (R37606). Cells were imaged on an M7000 (AMF7000) using an Olympus 20X objective (AMEP4734) with DAPI (AMEP4650), GFP (AMEP4651) and RFP (AMEP4652) LED cubes.
These neurons differ in the rat strain from which they were isolated: Sprague Dawley vs. Fisher 344. These neurons don't expand anymore. They just grow neurites. The manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/MAN0001574-PrimaryRatCortex-HippocampusNeurons-UG.pdf) has instructions for culturing the cells. You can use Neurobasal Plus/B-27 Plus/GlutaMAX to culture them, or just Neurobasal/B-27/GlutaMAX. However, the former combination provides much superior performance than the latter and can maintain neurons longer.
We recommend using the B-27 Plus Neuronal Culture System when culturing neurons on Poly-D-Lysine. The B-27 Plus Neuronal Culture System is a complete media system that includes the B-27 Plus Supplement and the Neurobasal Plus Medium.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
The Poly-D-Lysine matrix has successfully supported the attachment of primary rodent neurons, iPSC-derived neurons and PC12 cells.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Primary rat neurons cannot be cryopreserved again because they will not revive.
No, primary rat neurons do not proliferate.
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