TCBS Cholera Medium (Dehydrated)

Selectively isolate pathogenic Vibrio species with Thermo Scientific™ Oxoid™ TCBS (Thiosulfate Citrate Bile Salts Sucrose) Cholera Medium (Dehydrated). The medium has shown to be the most satisfactory and reproducible formula regardless of the complex composition¹. Unlike Lauryl Sulphate Tellurite Agar it does not require further additives or aseptic additions of blood. It also possesses superior growth characteristics for Vibrio species, compared with tellurite media. While inhibiting non-vibrios, it promotes rapid growth of pathogenic vibrios after overnight incubation at 35°C.

Vibrio is a comma-shaped Gram-negative bacteria. Vibrios are aquatic microorganisms, some species of which cause serious diseases in humans and other animals. Three species of Vibrio are of significance to humans: Vibrio cholerae is the cause of cholera, Vibrio parahemolyticus and V. vulnificus both act as agents of acute enteritis, or bacterial diarrhea.

Use TCBS Cholera Medium for selective isolation of pathogenic vibrios.

• Produces reproducible results.
• No need for additives or aseptic additions of blood.
• Promotes rapid growth of pathogenic vibrios.

The TCBS medium conforms to the formulation of Kobayashi et al., except that it contains specially processed ox bile, free from the defects noted by Nakanishi and Kobayashi².

The TCBS medium is suitable for growth of Vibrio cholerae, Vibrio parahemolyticus, and most other Vibrios³. Most Enterobacteriaceae encountered in feces are totally suppressed for at least 24 hours. Slight growth of Proteus species and Enterococcus fecalis may occur but the colonies are easily distinguished from Vibrio colonies.

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