Tris-Glycin Transferpuffer (25x)
Tris-Glycin Transferpuffer (25x)
Invitrogen™

Tris-Glycin Transferpuffer (25x)

Tris-Glycin-Transferpuffer (25X) ist im Rahmen eines Western Blots für die Übertragung von Proteinen aus Tris-Glycin-Gelen auf Nitrocellulose-, PVDF- oder NylonmembranenWeitere Informationen
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KatalognummerMenge
LC3675500 mL
Katalognummer LC3675
Preis (EUR)
84,25
Each
-
Zum Warenkorb hinzufügen
Menge:
500 mL
Preis (EUR)
84,25
Each
Zum Warenkorb hinzufügen
Tris-Glycin-Transferpuffer (25X) ist im Rahmen eines Western Blots für die Übertragung von Proteinen aus Tris-Glycin-Gelen auf Nitrocellulose-, PVDF- oder Nylonmembranen für die anschließende Analyse optimiert.

Anwendung: Dieser Puffer sollte mit einer Wasser-/Methanolmischung zu einer 1X-Lösung verdünnt werden, um eine endgültige Methanolkonzentration von 20 % für optimale Ergebnisse zu erzielen.

Alle verfügbaren Puffer und Reagenzien für SDS-PAGE anzeigen

Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.
Specifications
Konzentration25X
GeltypTris-Glycin-Gel
Menge500 mL
VersandbedingungRaumtemperatur
Gel CompatibilityNovex™ Tris-Glycin-Gel
Membrane CompatibilityNitrozellulose, PVDF, Nylon
ProduktlinieNovex™
ProdukttypTransferpuffer
Unit SizeEach
Inhalt und Lagerung
Bei Raumtemperatur lagern.

Häufig gestellte Fragen (FAQ)

What type of transfer buffer should be used with Invitrogen Tricine gels?

For non-sequencing applications, any transfer buffer used with Tris-Glycine gels can be used with Tricine gels including Tris-Glycine transfer buffer. For sequencing applications, the buffer should be chemically compatible with sequencing protocols. Non-glycine based transfer buffers such as the NuPAGE Transfer buffer, 1/2X TBE Transfer buffer, or CAPS Buffer can be used for N-terminal sequencing . Generally, a pH which is close to neutral is desirable to maintain gel and protein stability. High current should be avoided because it can lead to heat generation and instability.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

When blotting with the XCell II Blot Module, what would happen if I filled the outer buffer chamber with transfer buffer instead of water?

This is perfectly acceptable with the XCell II Blot Module. The liquid in the outer buffer chamber only serves as a coolant or heat sink. The reason why water is recommended is because it is a less expensive alternative.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

The pH of my transfer buffer deviates from the recommended value by 0.2 units. Can I still use the buffer?

We recommend discarding the buffer and remaking it after rechecking the reagents and the water purity. We do not recommend adjusting the pH with acid or base, as this will increase the conductivity of the buffer and result in higher current during the transfer.

Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.

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