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KnockOut™ Serum Replacement
KnockOut™ Serum Replacement
Gibco™

KnockOut™ Serum Replacement

KnockOut™ Serum Replacement
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Catalog NumberQuantity
10828028Promo Image500 mL
10828010Promo Image100 mL
Catalog number 10828028
Price (EUR)
462,65
Online Exclusive
482,00
Save 19,35 (4%)
Each
In stock
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Quantity:
500 mL
Price (EUR)
462,65
Online Exclusive
482,00
Save 19,35 (4%)
Each
Add to cart
Gibco™ KnockOut™ Serum Replacement (KnockOut™ SR) is a more defined, FBS-free medium supplement that supports the growth of pluripotent stem cells (PSCs) cultured on fibroblast feeder cells. Designed to replace fetal bovine serum (FBS) in existing protocols, this serum replacement has been trusted by many for almost 20 years. Providing the documents required for regulatory review, KnockOut SR reduces your burden in qualifying reagents when transitioning from research applications to clinical applications. If you are interested in only basic research applications, see the Research Use Only version of this product to ensure that you receive the benefits and pricing that fit your needs.

With KnockOut Serum Replacement, you can:
  • Culture feeder-dependent PSCs with confidence
  • Rely on the improved performance of a qualified, FBS-free formulation
  • Enjoy the peace of mind of having a more stable price and supply compared to FBS

    Culture PSCs with confidence
    KnockOut SR has been cited in more than 2000 publications. It has been shown to support feeder-dependent culture of embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). It has also been shown to support reprogramming, embryoid body (EB) formation, in vitro differentiation, cryopreservation, ESC derivation, and many other applications.

    Rely on improved performance
    KnockOut SR has more a defined formulation compared to FBS, which contains many undefined and variable components. Unlike most FBS products, each lot is tested for its ability to support PSCs. Because of this, culturing PSCs with KnockOut SR leads to less differentiation and more compact colony morphology compared to FBS.

    Enjoy the peace of mind of having stable price and supply
    KnockOut SR is not affected by the limited supply and fluctuating prices often seen with FBS. This makes it easier to plan your budget and buy what you need, especially since it is more affordable than most FBS products.



    This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in a specific region, it should be used for laboratory use only.
  • Specifications
    Cell TypeStem Cells (Embryonic),Stem Cells (iPS - Induced Pluripotent Stem)
    ClassificationSerum-Free
    Culture TypeStem Cell (Embryonic, iPS - Induced Pluripotent Stem)
    For Use With (Application)Clinical Research, Translational Research
    Manufacturing QualitycGMP for medical devices, 21 CFR Part 820 and ISO 13485
    Product LineKnockOut™
    Serum LevelSerum-Free
    Shelf Life18 Months
    Shipping ConditionDry Ice
    FormFrozen
    Product TypeSerum Replacement
    Quantity500 mL
    pH6 to 8
    Unit SizeEach
    Contents & Storage
    Store in freezer (-5 to -20°C) and protect from light.

    Frequently asked questions (FAQs)

    Can KnockOut SR be used to culture mouse ESCs in feeder-free conditions?

    Yes, mouse embryonic stem cells can be cultured using KnockOut SR under feeder-free conditions. Typically, cells are plated at a higher seeding density, in the presence of LIF, on a 0.1% gelatin layer in place of feeder cells.

    Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

    Can KnockOut SR be used to culture mouse ESCs on feeder cells?

    Yes, KnockOut SR was designed to culture mouse ES cells in undifferentiated conditions.

    Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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