CD Hybridoma Medium
CD Hybridoma Medium
Gibco™

CD Hybridoma Medium

CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growthRead more
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Catalog NumberQuantity
112790231000 mL
Catalog number 11279023
Price (EUR)
176,00
Each
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Quantity:
1000 mL
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Price (EUR)
176,00
Each
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CD Hybridoma Medium is an animal origin–free, chemically defined, protein-free medium specifically developed for the ability to support the growth of hybridomas for antibody production. Gibco™ CD Hybridoma Medium features:

• Ability to support suspension and stationary hybridomas and myelomas
• Formulated without L-glutamine for stability
• Animal origin–free, protein-free, and chemically defined formulation

Ability to support suspension and stationary hybridomas and myelomas
Suitable for the culture of recombinant myeloma lines as well as traditional hybridomas, Gibco™ CD Hybridoma Medium provides high cell densities and IgG production. Cholesterol-dependent cultures such as NS0 require the addition of 250X Cholesterol Lipid Concentrate.

Formulated without L-glutamine for stability
Gibco™ CD Hybridoma Medium requires the addition of 8 mM L-glutamine or GlutaMAX™ supplement before use. GlutaMAX™ supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format.

Animal origin–free, protein-free, and chemically defined formulation
Gibco™ CD Hybridoma Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest. Gibco™ chemically defined media contain no proteins, hydrolysates, or components of unknown composition.

Product use
Customers using Gibco™ CD Hybridoma Medium in a manufacturing process, who have a submission with the FDA, may request from us a letter of authorization to reference our Type II Drug Master File (DMF).

cGMP manufacturing and quality system
Gibco™ CD Hybridoma Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell TypeMyeloma Cells, Hybridomas
Product LineGibco™
Product TypeHybridoma Medium
Quantity1000 mL
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free, Chemically-defined, Protein-free
FormLiquid
Without AdditivesNo Glutamine, No Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: 2-8°C. Protect from light
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
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Frequently asked questions (FAQs)

How do I adapt my cells to serum-free medium?

Cells can be adapted by Sequential or Direct Adaptation. Suggested protocols for each are below, and you can also find more information by searching "Adaptation of Cell Cultures to a Serum-Free Medium" from our website home page.

SEQUENTIAL ADAPTATION
1) Subculture the cells growing in serum-supplemented medium into a 25%:75% mixture of SFM and serum supplemented medium.
2) When the cell density is 5 x 10E5 cells/ml, subculture the cells into a 50%:50% mixture of SFM and serum supplemented medium at a cell density 2.5 x 10E5 to 3 x 10E5 cells/ml.
3) Continue to subculture after the cell density 5 x 10E5 cells/ml in gradually increasing proportions of SFM until the serum is ~0.1% with about 85% cell viability.
4) Subculture the cells into SFM with an innoculum of 2.5 x 10E5 to 3 x 10E5 cells/ml.
5) When the cell density is 1 x 10E6 to 3 x 10E6 cells/ml (4 to 6 days post planting) subculture the cells again.
6) Stock cultures of SFM adapted cells should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

DIRECT ADAPTATION
Some cells can be directly adapted from serum-containing medium to SFM. For direct adaptation, the cell innoculum should be 1.5 x 10E5 to 3 x 10E5 cells/ml.
Cells should be subcultured when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml. Cells are fully adapted to SFM when the cell density is 2 x 10E6 to 4 x 10E6 cells/ml after 4 to 7 days in culture.
Stock cultures of cells adapted to SFM should be subcultured in SFM every 3 to 5 days when the cell density is 1 x 10E6 to 3 x 10E6 cells/ml with 90% viability.

Why is it necessary to gradually adapt the cells to serum-free medium?

Some cells, such as insect cells, are sensitive to changes in their medium. By sequentially adapting cells, the medium is changed with minimal effects on cell growth.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I filter sterilize CD Hybridoma Medium?

CD Hybridoma Medium can be filtered by 0.2 µm filtration.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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