Leibovitz's L-15 Medium
Leibovitz's L-15 Medium
Gibco™

Leibovitz's L-15 Medium

Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue.Read more
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Catalog NumberQuantity
11415064500 mL
Catalog number 11415064
Price (EUR)
31,65
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36,37
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Quantity:
500 mL
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Price (EUR)
31,65
Online Exclusive
36,37
Save 4,72 (13%)
Each
Add to cart
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Leibovitz's L-15 Medium supports the growth of HEP-2 monkey kidney cells and primary explants of embryonic and adult human tissue. L-15 is buffered by phosphates and free base amino acids instead of sodium bicarbonate. This medium is designed for supporting cell growth in environments without CO2 equilibration.


This L-15 is modified as follows:
WithWithout
• Galactose• Glucose
• Phenol Red• HEPES
• L-glutamine• Sodium Bicarbonate
• Sodium Pyruvate 


The complete formulation is available.

L-15 contains no proteins, lipids, or growth factors. Therefore, F-10 requires supplementation, commonly with 10% Fetal Bovine Serum (FBS).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Cell LineHEP-2 monkey kidney cells
Cell Typeexplants of embryonic and adult human tissue
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Product TypeLeibovitz's L-15 Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionRoom Temperature
ClassificationAnimal Origin
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutamine, Phenol Red, Sodium Pyruvate, Galactose
Without AdditivesNo Glucose, No HEPES, No Sodium Bicarbonate
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light
Shipping conditions: Ambient
Shelf life: 12 months from date of manufacture
Media Formulations
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Frequently asked questions (FAQs)

What is the osmolality of Leibovitz's L-15 Medium?

We do provide osmolality information on the certificate of analysis. All lots of Leibovitz's L-15 Medium will meet the osmolality specification of 300-340 mOsm/kg.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What are the Leibovitz media you offer?

We offer the following Leibovitz media:

- Leibovitz's L-15 Medium, powder: Cat. Nos. 41300039 (10x 1L) and 41300070 (1x 10L)
- Leibovitz's L-15 Medium, no phenol red: Cat. No 21083027 (1x 500 mL)
- Leibovitz's L-15 Medium: Cat. Nos.11415064 (1x 500 mL) and 11415114 (10x 500 mL) (for sale in N. America, Latin America, and Asia-Pacific regions; Cat. No. 11415049 (for sale in Europe, Africa, and the Middle East
- Leibovitz's L-15 Medium with GlutaMAX Supplement: Cat. No. 31415029 (1x 500 mL)

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How long can I keep my media after supplementing with serum?

Generally speaking, media can be used for up to three weeks after supplementation with serum. There are no formal studies to support this, but it is the rule of thumb used by our scientists.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

My medium was shipped at room temperature but it is supposed to be stored refrigerated. Is it okay?

We routinely ship media that require long-term storage in the refrigerator at room temperature. We have done studies on representative media formulations to show that media can be at room temperature for up to a week without a problem.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How can I remove mycoplasma contamination from my cell culture medium?

Very often mycoplasma contamination cannot be removed from the culture so it should be discarded. You may have a unique culture that you prefer not to discard and would like to try to clean it. Ciprofloxacin and Plasmocin have reportedly been used for this application. If interested in a protocol or directions for use, check with the antibiotic supplier or published literature. Note that mycoplasma are very difficult to remove from culture and spread easily so the treated cultures should be quarantined until clear of mycoplasma, and your laboratory should be thoroughly cleaned.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3142789Certificate of AnalysisJun 18, 202511415064, 11415049
3228074Certificate of AnalysisJun 13, 202511415064, 11415049
3150467Certificate of AnalysisMay 24, 202511415064, 11415049
3092656Certificate of AnalysisApr 05, 202511415064, 11415049
3137161Certificate of AnalysisMar 30, 202511415064, 11415049
5 results displayed, search above for a specific certificate

Safety Data Sheets

Citations & References (3)

Citations & References
Abstract
Tumor necrosis factor alpha increases the expression of glycosyltransferases and sulfotransferases responsible for the biosynthesis of sialylated and/or sulfated Lewis x epitopes in the human bronchial mucosa.
Authors: Delmotte Philippe; Degroote Sophie; Lafitte Jean-Jacques; Lamblin Geneviève; Perini Jean-Marc; Roussel Philippe;
Journal:J Biol Chem
PubMed ID:11679593
'There is increasing evidence that inflammation may affect glycosylation and sulfation of various glycoproteins. The present study reports the effect of tumor necrosis factor alpha (TNF-alpha), a proinflammatory cytokine, on the glycosyl- and sulfotransferases of the human bronchial mucosa responsible for the biosynthesis of Lewis x epitope and of its ... More
Antiapoptotic activity of the free caspase recruitment domain of procaspase-9: a novel endogenous rescue pathway in cell death.
Authors: Stephanou Anastasis; Scarabelli Tiziano M; Knight Richard A; Latchman David S;
Journal:J Biol Chem
PubMed ID:11825888
Mitochondrial injury initiates proteolytic processing of procaspase-9 into the large and small subunits, leading to apoptotic cell death. Here we show that the free caspase recruitment domain (CARD) released by procaspase-9 processing activates nuclear factor kappaB expression. A procaspase-9 construct with a point mutation that abrogates the release of the ... More
Expression and localization of aquaporin water channels in rat hepatocytes. Evidence for a role in canalicular bile secretion.
Authors: Huebert Robert C; Splinter Patrick L; Garcia Fabiana; Marinelli Raul A; LaRusso Nicholas F;
Journal:J Biol Chem
PubMed ID:11932260
Although bile formation requires that large volumes of water be rapidly transported across liver epithelia, including hepatocytes, the molecular mechanisms by which water is secreted into bile are obscure. The aquaporins are a family of 10 channel-forming, integral membrane proteins of approximately 28 kDa numbered 0-9 that allow water to ... More
3 total citations

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