The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombinationRead more
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Catalog number 12283016
also known as 12283-016
Price (EUR)/ 6 µg
890,80
Online exclusive
1048.00
Save 157,20 (15%)
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Add to cart
Price (EUR)/ 6 µg
890,80
Online exclusive
1048.00
Save 157,20 (15%)
Add to cart
pBAD-DEST49 Gateway™ Destination Vector
Catalog number12283016
Price (EUR)/ 6 µg
890,80
Online exclusive
1048.00
Save 157,20 (15%)
-
Add to cart
The pBAD-DEST49 Gateway™ destination vector is designed for rapid cloning with a Gateway™ entry clone using lambda phage site-specific recombination and subsequent high-level, tightly regulated expression in E. coli. The pBAD-DEST49 vector features:
• The araBAD promoter for tightly regulated expression in E. coli • HP-thioredoxin fusion for improved protein yield and solubility • Enterokinase cleavage site for efficient cleavage of the N-terminal fusion with EKMax™ • C-terminal V5 and 6xHis tags for efficient detection and purification of fusion proteins • R sites for efficient recombination with any attL-flanked Gateway™ entry vector
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemInducible
Inducing AgentArabinose
PromoteraraBAD
Product TypeGateway System Destination Expression Vector
Selection Agent (Eukaryotic)None
Antibiotic Resistance BacterialAmpicillin (AmpR)
CleavageEK (Enterokinase) Recognition Site
Protein TagHis Tag (6x), V5 Epitope Tag, Thioredoxin
Cloning MethodGateway
Quantity6 μg
VectorpBAD, pDEST
Product LineGateway™
Unit Size6 µg
Contents & Storage
The pBAD-DEST49 Gateway™ destination vector includes 6 μg of vector. Store at -20°C. The vector is guaranteed stable for 6 months when properly stored.