Recovery™ Cell Culture Freezing Medium
Recovery™ Cell Culture Freezing Medium
Recovery™ Cell Culture Freezing Medium
Recovery™ Cell Culture Freezing Medium
Gibco™

Recovery™ Cell Culture Freezing Medium

Recovery Cell Culture Freezing Medium is a complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells,Read more
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Catalog NumberQuantity
1264801050 mL
Catalog number 12648010
Price (EUR)
189,00
Each
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50 mL
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Price (EUR)
189,00
Each
Add to cart
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Recovery Cell Culture Freezing Medium is a complete, ready-to-use freezing medium for cryopreservation of a wide variety of mammalian cells, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3. Recovery Cell Culture Freezing Medium features:

• Classic formulation optimized for better performance
• Suitability for a wide variety of mammalian cells
• Quality and performance tested

Classic formulation optimized for better performance
For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using high-quality Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

Suitability for a wide variety of mammalian cells
Recovery Cell Culture Freezing Medium can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham's F-12, including CHO-S, CHO-K1, HEK 293, Jurkat, and NIH 3T3 cells.

Quality and performance tested
Recovery Cell Culture Freezing Medium is quality tested for pH, osmolality, sterility, and endotoxin. In addition, each lot is performance tested using CHO-K1 cells.

cGMP manufacturing and quality system
For supply chain continuity, we manufacture Gibco Recovery Cell Culture Freezing Medium at two separate facilities located in Grand Island, NY and Scotland, UK. Both sites are compliant with cGMP manufacturing requirements and are certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Product LineGibco™, Recovery™
Product TypeFreezing Medium
Purity or Quality GradeResearch Grade
Quantity50 mL
Shelf Life12 Months
Shipping ConditionDry Ice
Culture TypeMammalian Cell Culture
FormLiquid
Serum LevelStandard Serum Supplementation
SterilitySterile-filtered
With AdditivesHigh Glucose, Phenol Red, DMSO (10%)
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: -5 to -20°C. Protect from light.
Shipping conditions: Frozen
Shelf life: 12 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3011479Certificate of AnalysisJun 15, 202512648010
3011467Certificate of AnalysisMay 29, 202512648010
3011447Certificate of AnalysisMay 01, 202512648010
3108132Certificate of AnalysisApr 13, 202512648010
3011431Certificate of AnalysisApr 04, 202512648010
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Safety Data Sheets

Frequently asked questions (FAQs)

Mammalian cells are cryopreserved to avoid loss by contamination, to minimize genetic change in continuous cell lines, and to avoid aging and transformation in finite cell lines. Before cryopreservation, cells should be characterized and checked for contamination.

There are several common media used to freeze cells. For serum-containing medium, the constituents may be as follows:
1) Complete medium containing 10% DMSO (dimethylsulfoxide)
2) 50% cell-conditioned medium with 50% fresh medium with 10% DMSO

Cryopreservation media generally consists of a base medium, cryopreservative, and a protein source. The cryopreservative and protein protect the cells from the stress of the freeze-thaw process. A serum-free medium has generally low or no protein, but one can still use it as a base for a cryopreservative medium in the following formulations:

1) 50% cell-conditioned serum free medium and 50% fresh serum-free medium containing 7.5% DMSO
2) Fresh serum-free medium containing 7.5% DMSO and 10% cell culture grade BSA

Protocol for Suspension Cultures:
1. Count the number of viable cells to be cryopreserved. Cells should be in log phase.
2. Centrifuge the cells at ~200 to 400 x g for 5 min to pellet cells.
3. Using a pipette, remove the supernatant down to the smallest volume without disturbing the cells.
4. Resuspend cells in freezing medium to a concentration of 1 x 10E7 to 5 x 10E7cells/ml for serum-containing medium, or 0.5 x 10E7to 1 x 10E7 cells/ml for serum-free medium.
5. Aliquot into cryogenic storage vials. Place vials on wet ice or in a 4°C refrigerator, and start the freezing procedure within 5 minutes.
6. Cells are frozen slowly at 1°C /min. This can be done by programmable coolers or by placing vials in an insulated box placed in a -70°C to -90°C freezer, then transferring to liquid nitrogen storage.

Protocol for Adherent Cultures:
1. Detach cells from the substrate with dissociation agents. Detach as gently as possible to minimize damage to the cells.
2. Resuspend the detached cells in a complete growth medium and establish the viable cell count.
3. Centrifuge at ~200 x g for 5 min to pellet cells.
4. Using a pipette, withdraw the supernatant down to the smallest volume without disturbing the cells.
5. Resuspend cells in freezing medium to a concentration of 5 x 10E6 to 1 x 10E7 cells/ml. Aliquot into cryogenic storage vials.
6. Place vials on wet ice or in a 4°C refrigerator, and start the freezing procedure within 5 min.
7. Cells are frozen slowly at 1°C /min. This can be done by programmable coolers or by placing vials in an insulated box placed in a -70°C to -90°C freezer, then transferring to liquid nitrogen storage.
Reference: Freshney, R. (1987) Culture of Animal Cells: A Manual of Basic Technique, p. 220, Alan R. Liss,Inc., New York.

Thawing of Cryopreserved Cells:
Cryopreserved cells are fragile and require gentle handling. Thaw cells quickly and plate directly into complete growth medium. If cells are particularly sensitive to cryopreservation (DMSO or glycerol), they are centrifuged to remove cryopreservative and then plated into growth medium. The following are suggested procedures for thawing cryopreserved cells:

- Centrifugation Method: Remove cells from storage and thaw quickly in a 37°C water bath. Place 1 or 2 ml of frozen cells in ~25 ml of complete growth medium. Mix very gently. Centrifuge cells at ~80 x g for 2 to 3 min. Discard supernatant. Gently Resuspend cells in complete growth medium and perform a viable cell count. Plate the cells. Cell innoculum should be at least 3 x 10E5 cells/ml.
- Direct Plating Method: Remove cells from storage and thaw quickly in a 37°C water bath. Plate cells directly with complete growth media.

Our Cell Culture Freezing Media is composed of DMEM, FBS, calf serum, and 10% DMSO. This is useful for many mammalian cells for freezing and is the same percentage of DMSO used in most methods. This product will work fine for most adherent cell lines grown with serum. A good general protocol for freezing cells can be found on our website by searching "Freezing cells protocol" from the home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Recovery Cell Culture Freezing Medium is meant for general cell culture applications where a wide range of growth media will be used. We recommend that you aspirate Recovery Cell Culture Freezing Medium before seeding, although other protocols can be substituted (this will need to be determined by the end user).

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Recovery Cell Culture Freezing Medium should be stable at 2 to 8 degrees C for 1 week, although we have no data to support this.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

There have not been any bench studies at this time. It is best to aliquot this product.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Citations & References (1)

Citations & References
Abstract
Sleeping Beauty transposon mutagenesis of the rat genome in spermatogonial stem cells.
Authors:Ivics Z, Izsvák Z, Chapman KM, Hamra FK,
Journal:Methods
PubMed ID:21193047
'Since several aspects of physiology in rats have evolved to be more similar to humans than that of mice, it is highly desirable to link the rat into the process of annotating the human genome with function. However, the lack of technology for generating defined mutants in the rat genome ... More
1 total citations

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