HBSS, calcium, magnesium, no phenol red
HBSS, calcium, magnesium, no phenol red
Gibco™

HBSS, calcium, magnesium, no phenol red

Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation,Read more
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Catalog NumberQuantity
14025092500 mL
Catalog number 14025092
Price (EUR)
42,76
Each
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Quantity:
500 mL
Customize this product
Price (EUR)
42,76
Each
Add to cart
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Hanks' Balanced Salt Solution (HBSS) is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. Formulations with calcium and magnesium are generally used as transport media, or for reagent preparation.

We offer a variety of Gibco™ HBSS formulations for a range of cell culture applications.


This HBSS is modified as follows:
WithWithout
• Calcium• Phenol Red
• Magnesium• Sodium Pyruvate
• Glucose 


The complete formulation is available.

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
AdditiveNo Sodium Pyruvate
ClassificationAnimal Origin-Free
ColorClear
Dilution1 X
For Use With (Application)Mammalian Cell Culture
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality270 - 305 mOsm/kg
Phenol Red IndicatorNo Phenol Red
Product LineGibco™
Quantity500 mL
Shipping ConditionRoom Temperature
SterilitySterile-filtered
FormLiquid
Product TypeHanks' Balanced Salt Solution (HBSS)
Solution TypeHank's Balanced Salt
pH6.7 - 7.8
Unit SizeEach
Contents & Storage
Storage conditions: 15–30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3150761Certificate of AnalysisJun 22, 202514025076, 14025050, 14025092
3148763Certificate of AnalysisJun 11, 202514025076, 14025050, 14025092
3209557Certificate of AnalysisJun 07, 202514025076, 14025050, 14025092
3140604Certificate of AnalysisJun 06, 202514025076, 14025050, 14025092
3150543Certificate of AnalysisMay 11, 202514025076, 14025050, 14025092
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Safety Data Sheets

Frequently asked questions (FAQs)

The recommended storage condition for our HBSS products is at 15-30 degrees C. Generally, storage at 4 degrees C will not impact these products. The only concern would be for the 10x HBSS solutions with calcium and magnesium as these salts might precipitate when stored outside the recommended temperature ranges. As long as you don't see any precipitation upon refrigeration, the product will be fine.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

HBSS (Cat. No. 14025xxx) does not contain HEPES and it needs to be externally supplemented. The complete formulation is available here (https://www.thermofisher.com/us/en/home/technical-resources/media-formulation.153.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Most media contain phenol red, which can quench fluorescent dyes in the visible wavelengths. Most media also contain autofluorescent components, such as riboflavin, which can reduce signal-to-background. We offer FluoroBrite DMEM and HEPES-based Live Cell Imaging Solution, which have been optimized for fluorescent imaging. We also offer a number of media without phenol red. But if none of these are reasonable options for your experiment, then we also offer BackDrop Background Suppressor ReadyProbes Reagent, which can be added to quench media autofluorescence.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Some cell types accumulate phenol red, and this can pose a problem in the use of many fluorescent probes. Phenol red can quench visible-wavelength dyes and, although phenol red is non-fluorescent, various impurities may be fluorescent. We have many phenol red-free media to choose from. Our Live Cell Imaging Solution (HEPES-based) and our FluoroBrite DMEM have been optimized to be phenol red-free as well as to be non-autofluorescent.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Phenol red is a pH indicator which allows you to determine the optimal atmospheric conditions for your cell line during your particular assays. We offer both versions to allow you the option to utilize this indicator in your media.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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