Embryonic Stem Cell FBS, qualified, US origin
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Embryonic Stem Cell FBS, qualified, US origin
Gibco™

Embryonic Stem Cell FBS, qualified, US origin

Fetal Bovine Serum, ES cell qualified, is specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells.
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Catalog NumberQuantityFormat
16141079Promo Image500 mLBottle
1614100250 mLOne Shot
Catalog number 16141079
Price (EUR)
1.022,00
Each
In stock
Add to cart
Quantity:
500 mL
Format:
Bottle
Price (EUR)
1.022,00
Each
Add to cart
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Fetal Bovine Serum, ES cell qualified, is specially tested for the ability to sustain undifferentiated cellular morphology of embryonic stem cells. It is Gibco FBS quality and performance you trust offered in a single-use FBS aliquot, which provides greater convenience, saves time, and is easy to use.

  • Sera Category: Specialty
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Purity or Quality GradeES Cell-Qualified
Shipping ConditionFrozen
SpeciesCattle/Bovine
AgeFetal
Country of OriginUnited States
FormLiquid
FormatBottle
Product TypeFetal Bovine Serum
Quantity500 mL
Serum TreatmentStandard (Sterile-filtered)
SterilitySterile
Unit SizeEach
Contents & Storage
Storage conditions: ≤-10°C
Shipping conditions: Frozen
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Frequently asked questions (FAQs)

What are the benefits and disadvantages of using heat-inactivated FBS in cell culture?

  1. Heating inactivates complement. Active complement can participate in cytolytic events, contract smooth muscle, release histamine from mast cells and platelets, and activate lymphocytic and macrophage cells. Applications where heat-inactivated serum is recommended include immunological studies and culturing of embryonic stem cells (ESCs), insect cells, and smooth muscle cells.
  2. Heat inactivation helps to achieve bottle-to-bottle and lot-to-lot stability by neutralizing many factors that can vary largely from lot to lot.
  3. There aren't necessarily disadvantages to heat inactivation of FBS, but there is some evidence that suggests there may be no added benefit to it unless you are carrying out immune studies.

Note: Heat inactivation is performed in a 56 degrees C water bath for 30 min with swirling every 10 min or so for heat distribution and to lower the degree of protein aggregation/flocculant precipitation. Note: If the time or temperature is exceeded, the serum may thicken to a gel. If this occurs, the serum is no longer usable. Unnecessary heat inactivation can take up time and potentially lead to wasted reagents if a mistake is made during the protocol1.

1. Pellerin, et al., Bioengineering, published in 2021.

Find additional tips, troubleshooting help, and resources within our Mammalian Cell Culture Basics Support Center.

How long can I store Gibco Fetal Bovine Serum at 4 degrees C?

This product can be stored at 4 degrees C for up to 4 weeks. Once the medium has been supplemented with serum, we recommend using it within 2-4 weeks.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How should I thaw Gibco Fetal Bovine Serum?

We recommend thawing the serum overnight at 4 degrees C or in a 37 degrees C water bath, removing as soon as it is thawed. Once thawed, aliquot into single-use sizes and freeze the aliquots. Each aliquot should ideally be thawed only one additional time as repeated freeze-thaw cycles are not recommended.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How can I minimize lot-to-lot variability in serum?

You can test each new lot to be sure that it works for your specific applications. Alternatively, our serum sales team can determine the most consistent, highest-performing serum lot available using the Gibco iMatch Sera Lot Matching Tool.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I aliquot and refreeze serum?

Yes, after the overnight thaw, you can aliquot the serum into smaller, single-use aliquots and refreeze it.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

View all Embryonic Stem Cell FBS, qualified, US origin FAQs

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