N-2 Supplement (100X)

Gibco™

N-2 Supplement (100X)

N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.

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Catalog Number	Quantity
17502048	5 mL
17502001	50 mL

2 Options

Catalog number 17502048

Price (EUR)

105,00

Each

Add to cart

Quantity:

5 mL

Price (EUR)

105,00

Each

Add to cart

Gibco N-2 Supplement is a chemically defined, serum-free supplement based on Bottenstein's N-1 formulation, containing optimized concentrations of insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite. N-2 Supplement is recommended for growth and expression of neuroblastomas and neural cells in primary cultures from both the peripheral nervous system (PNS) and the central nervous system (CNS).

N-2 Supplement has been demonstrated in wide-ranging applications, such as the culture of neural stem cells, the culture of the SH-SY5Y cell line (subline of the neuroblastoma cell line SK-N-SH), and culture of the HT22 mouse hippocampal neuronal cell line. Additionally, N-2 Supplement adds support to cell culture media used to differentiate pluripotent stem cells (PSCs) to a variety of cell types, including neural and pancreatic-like cell types.

N-2 Supplement may be used with Neurobasal media supplemented with growth factors such as bFGF and EGF. When combined with Dulbecco's Modified Eagle Medium (D-MEM) and fetal bovine serum (FBS), N-2 Supplement also provides suitable supplementation for the culture of human and rodent astrocytes.

Each lot is cGMP manufactured
Each lot is tested for sterility, endotoxin, and performance
Contains insulin, iron-rich holo-transferrin, progesterone, putrescine, and selenite
Can be used as a substitute for Bottenstein's N-1 formulation
Provided as a 100X concentrate

Choose Gibco N-2 Supplement for a reliable, high-quality product trusted by researchers worldwide.

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell TypeNeural Cells

Concentration100 X

Culture TypeMammalian Cell Culture

Quantity5 mL

Serum LevelSerum-Free

Shelf Life18 Months

Shipping ConditionDry Ice

Tested ForEndotoxin, Performance, Sterility

ClassificationSerum-free

FormLiquid

Product TypeCell Culture Supplement

SterilitySterile

pH6 to 8

Unit SizeEach

Contents & Storage

Store in freezer (-5 to -20°C) and protect from light.

Media Formulations

N-2 Supplement (100X) liquid

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Frequently asked questions (FAQs)

What supplements should I use with Neurobasal or Neurobasal-A medium?

Neurobasal and Neurobasal-A media (for postnatal and adult neurons) allow for long-term maintenance of neuronal cells without the need for an astrocyte feeder layer. These media should be supplemented with either serum or a serum-free supplement, plus 0.5mM L-glutamine. B-27 supplement is a serum-free supplement that comes as a 50X concentrate in a 10ml volume. This is enough supplement for 500ml of media. Fetal, postnatal, and adult neural cultures can be grown in the appropriate Neurobasal medium supplemented with B-27 supplement .

We also have two other supplements. One is called G-5 and is for growth and expression of glial cells (normal and tumor) of astrocytic phenotype. This comes in a 1ml size, at a 100X concentration. The other supplement is called N-2 and is for growth and expression of post-mitotic neurons and tumor cells of neuronal phenotype. This comes in a 5ml size, at a 100X concentration.

For more information on Neurobasal media, search "Neurobasal" from our website home page.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Is there a difference between the Gibco N-2 Supplement formulation from Thermo Fisher and the N-2 Supplement formulation from Global Stem?

Both N-2 Supplement formulations can be used in the culture of neurons, but the concentrations of components in the formula for the Global Stem N-2 Supplement are higher than the Gibco N-2 Supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Can I filter the N-2 Supplement?

The N-2 Supplement (100X) solution can be filtered. We do not recommend filtering the medium once N-2 has been added at a final concentration of 1X.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

What is the recommended storage condition and shelf life for N-2 Supplement (100X) (Cat. No. 17502001 or 17502048)?

The shelf life for N-2 Supplement (100X) is 18 months from the date of manufacture when stored at the recommended storage condition of -5 to -20 degrees C, protected from light. The expiration date should be listed on the product label as well as on the COA for the lot of the supplement.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Which supplement can I add to Neurobasal Medium to culture my neural cells?

As a general guideline, we recommend using Gibco B-27 Plus Supplement to culture neural stem cells, hippocampal, and other CNS neurons.

- For neuroblastomas, or post-mitotic neurons from both PNS and CNS, Gibco N-2 Supplement can be used.
- For primary glial cells (astrocytes) or tumor cell lines of astrocytic phenotype (astrocytes and gliomas), or oligodendrocytes, Gibco G-5 Supplement can be used.