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Gibco™
Neurobasal™ Medium
Neurobasal Medium is a basal medium designed for long-term maintenance and maturation of pure pre-natal and embryonic neuronal cell populationsRead more
| Catalog Number | Quantity |
|---|---|
| 21103049 | 500 mL |
Catalog number 21103049
Price (EUR)
86,50
Each
In stock
Quantity:
500 mL
Delivery information
Standard Products - 1-2 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 2-3 business days for 2nd-tier cities, and 4 business days for 3rd-tier cities and remote areas.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Air Freight Restricted Products - 2-4 business days for Beijing, Shanghai, Guangzhou, Shenzhen and provincial capitals, 3-5 business days for 2nd-tier cities, and 6-10 business days for 3rd-tier cities and remote areas.
Supply Center: Delivered from the Supply Center closest to you.
Price (EUR)
86,50
Each
Neurobasal Medium is a basal medium designed for long-term maintenance and maturation of pure pre-natal and embryonic neuronal cell populations without the need for an astrocyte feeder layer when used with Gibco B-27 supplements.
Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.
For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
Neurobasal Medium is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of Neurobasal media and B-27 supplements.
For established protocols using Neurobasal media, access our Gibco Neurobiology Protocol Handbook.
View publications using Gibco neurobiology products at our Gibco CellCite.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeNeural Cells
Product LineNeurobasal™
Product TypeNeurobasal Medium
Quantity500 mL
Shelf Life12 Months From Date of Manufacture
Shipping ConditionAmbient
Tested ForEndotoxin, Performance, Sterility, Osmolality, pH
ClassificationSerum-free
Culture TypeNeuronal Cell Culture
FormLiquid
With AdditivesPhenol Red
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.
Media Formulations
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Lot #Certificate TypeDateCatalog Number(s)
3157167Certificate of AnalysisJun 26, 202521103049
3124491Certificate of AnalysisJun 06, 202521103049
3142746Certificate of AnalysisMay 15, 202521103049
3137184Certificate of AnalysisApr 19, 202521103049
3026675Certificate of AnalysisApr 02, 202521103049
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SDSScientific Resources
Product Information
Frequently asked questions (FAQs)
当神经细胞成熟时，L-谷氨酸盐具有兴奋毒性。对于原代海马神经元及其他种类的胚胎神经元而言，我们推荐您在初始种板的培养基中加入 25 µM L-谷氨酸盐。不过，4天之后就不应添加L-谷氨酸盐成份了，因为超过4天后L-谷氨酸盐对神经元细胞具有毒性。
对于神经母细胞瘤，L-谷氨酸盐可添加于种板的培养基及后续换液的培养基之中。
做为一般性的参考方案，我们推荐您使用Gibco B-27添加剂来培养神经干细胞，海马及其他类型的CNS神经元。
-对于来自PNS和CNS中的神经母细胞瘤或有丝分裂后的神经元，可添加Gibco N-2添加剂。
-对于原代胶质细胞（星形胶质细胞）或具有星形胶质细胞表型的肿瘤细胞系（星形胶质细胞和胶质细胞瘤），或少突胶质细胞，可使用Gibco G-5添加剂。
Neurobasal培养基专门针对胚胎神经元和出生前神经元的培养体系进行了优化。Neurobasal-A培养基专为培养出生后神经元与成年脑神经元进行了优化。这两类培养基仅在渗透压上有所区别。
Yes, the protein content in this product is high enough so filtering through a low protein binding filter as a 50X or 1X in solution should not be a problem.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
No, it is not recommended to freeze media due to the potential of precipitates forming upon thaw. Inorganic salts and amino acids in the formulation may come out of solution when exposed to temperature fluctuations. These precipitates will not go back into solution easily once formed.
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Citations & References (5)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
A comprehensive protocol for efficient differentiation of human NPCs into electrically competent neurons.
Journal:Journal of neuroscience methods
PubMed ID:39053772
Activation of Trk neurotrophin receptor signaling by pituitary adenylate cyclase-activating polypeptides.
Journal:J Biol Chem
PubMed ID:11784714
'Pituitary adenylate cyclase-activating polypeptide (PACAP), a neuropeptide that acts through G protein-coupled receptors, exerts neuroprotective effects upon many neuronal populations. However, the intracellular signaling mechanisms that account for PACAP''s trophic effects are not well characterized. Here we have tested the possibility that PACAP uses neurotrophin signaling pathways. We have found
Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.
Journal:J Neurobiol
PubMed ID:11920724
A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. Mouse cortical neurons were maintained in culture in serum-free medium (Neurobasal supplemented with B27) for 60 days in vitro (DIV). The levels of several proteins were evaluated by immunoblotting to demonstrate that these neurons
Axonal Transport Enables Neuron-to-Neuron Propagation of Human Coronavirus OC43.
Journal:J Virol
PubMed ID:29925652
Human coronaviruses (HCoVs) are recognized respiratory pathogens for which accumulating evidence indicates that in vulnerable patients the infection can cause more severe pathologies. HCoVs are not always confined to the upper respiratory tract and can invade the central nervous system (CNS) under still unclear circumstances. HCoV-induced neuropathologies in humans are
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids.
Journal:Cell Stem Cell
PubMed ID:32579880
SARS-CoV-2 has caused the COVID-19 pandemic. There is an urgent need for physiological models to study SARS-CoV-2 infection using human disease-relevant cells. COVID-19 pathophysiology includes respiratory failure but involves other organ systems including gut, liver, heart, and pancreas. We present an experimental platform comprised of cell and organoid derivatives from
5 total citations