Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Pierce™ Biotinylated Protein Interaction Pull-Down Kit
Thermo Scientific™

Pierce™ Biotinylated Protein Interaction Pull-Down Kit

The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of aRead more
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Catalog NumberQuantity
2111525 reactions kit
Catalog number 21115
Price (EUR)
680,00
Each
In stock
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Quantity:
25 reactions kit
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Price (EUR)
680,00
Each
Add to cart
The Thermo Scientific Pierce Biotinylated Protein Interaction Pull-Down Kit contains the necessary components to capture and purify interactors of a biotin-labeled protein or ligand.

Features of the Biotinylated Protein Interaction Pull-Down Kit:

• Provides a complete, affordable and easy-to-use strategy for discoverying protein:protein interactions
• Uses common laboratory equipment and reagents (e.g., microcentrifuges, mini-gels, protein stains)
• Adaptable to single- or multiple-sample demands
• Flexible pull-down format uses spin cups for easy and efficient manipulation of streptavidin agarose beads

You provide the biotinylated protein as the 'bait' (see

Related Products
EZ-Link™ Desthiobiotinylation and Pull-Down Kit
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayAffinity Purification
For Use With (Application)Pull-Down (Biotin-Based)
For Use With (Equipment)Microcentrifuge
FormatKit
Quantity25 reactions kit
Sufficient For25 Reactions
TargetNot Target-Specific
Product LinePierce™
TypePull-Down Kit
Unit SizeEach
Contents & Storage
Sufficient For: At least 25 pull-down reactions, each using 25 μL of resin
• Streptavidin Agarose Resin, 1.5 mL
• TBS Pack (makes 500mL), 1 pack
• Biotin Blocking Solution, 15 mL
• Wash Buffer (pH 5.0), 100 mL
• Elution Buffer (pH 2.8), 50 mL
• Pierce Spin Columns and Accessories, 27 columns
• Microcentrifuge Collection Tubes, 200 tubes

Store at 4°C

Frequently asked questions (FAQs)

If my protein is endogenously expressed, which Thermo Scientific Protein:Protein Interaction (PPI) kit should I choose?

We recommend the biotin kit for endogenously expressed proteins. The reason for this is the wide variety of biotinylation reagents that can be used to label the detection protein of the binding pair. For help choosing a biotinylation reagent, refer to the Biotinylation Selection Guide available at our website.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How is the tagged protein detected on the membrane using the Thermo Scientific Protein:Protein Interaction (PPI) kits?

The biotin kit utilizes streptavidin-HRP to detect the biotinylated protein bound to the prey protein on the membrane. The GST kit utilizes a polyclonal goat anti-GST antibody for detection of the glutathione-S-transferase tagged fusion protein bound to another protein on a membrane.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

How many reactions can be performed using one Thermo Scientific Protein:Protein Interaction (PPI) kit?

The Thermo Scientific Pull-Down Protein:Protein Interaction Kits contain a complete, validated set of reagents specifically developed for performing pull-down assays.The kits contain sufficient materials for conducting 25 pull-down assays.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Are all of the conditions for binding the prey protein to the bait protein described in the Thermo Scientific Protein:Protein Interaction (PPI) kits?

No. Every protein pair will require unique conditions in order to bind with one another. Factors to consider are pH, ionic strength of the buffer, need for cofactors, whether the interaction is long term or transient, etc. The researcher is recommended to perform a literature search or try several different conditions in order to determine what conditions are best for the binding of their proteins of interest. The kit includes a “universal“ wash buffer formed by diluting the lysis buffer 1:1 in TBS.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

With the Thermo Scientific Protein:Protein Interaction (PPI) kits, what methods can be used to detect the prey protein?

The prey protein can be detected following transfer on a membrane. The membrane procedures uses 1:100,000 - 1:500,000 dilution of the anti-GST antibody.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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