Polymyxin B Sulfate
Polymyxin B Sulfate
Gibco™

Polymyxin B Sulfate

Polymyxin B Sulfate is produced by Bacillus polymyxa. Polymyxin B Sulfate works by binding to the cellular membrane and interferingRead more
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Catalog NumberQuantity
2185002925 MU
Catalog number 21850029
Price (EUR)
163,00
Each
-
Add to cart
Quantity:
25 MU
Price (EUR)
163,00
Each
Add to cart
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Polymyxin B Sulfate is produced by Bacillus polymyxa. Polymyxin B Sulfate works by binding to the cellular membrane and interfering with its permeability. Gibco™ Polymyxin B Sulfate is used for the prevention of bacterial contamination of cell cultures. This antibiotic is highly active against gram-negative bacteria. The recommended working concentration is 100 units/ml. We offer a variety of antibiotics and antimycotics for cell culture applications.

Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration100 Units/mL
For Use With (Application)Bacterial Selection
Quantity25 MU
Shelf Life24 Months
Shipping ConditionRoom Temperature
FormPowder
Product TypeAntibiotic
SterilityNon-sterile
Unit SizeEach
Contents & Storage
Storage conditions: 15 to 30°C
Shipping conditions: Ambient
Shelf life: 24 months from date of manufacture
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Documents & Downloads

Certificates

Lot #Certificate TypeDateCatalog Number(s)
3095031Certificate of AnalysisFeb 20, 202521850029
2686642Certificate of AnalysisJan 29, 202521850029
2195529Certificate of AnalysisJan 29, 202521850029
2068557Certificate of AnalysisJan 29, 202521850029
2965702Certificate of AnalysisJan 25, 202521850029
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Safety Data Sheets

Scientific Resources

Frequently asked questions (FAQs)

When an irreplaceable culture becomes contaminated, researchers may attempt to eliminate or control the contamination.

1. Determine if the contamination is bacteria, fungus, mycoplasma, or yeast. Read more here to view characteristics of each contaminant.
2. Isolate the contaminated culture from other cell lines.
3. Clean incubators and laminar flow hoods with a laboratory disinfectant, and check HEPA filters.
4. Antibiotics and antimycotics at high concentrations can be toxic to some cell lines. Therefore, perform a dose-response test to determine the level at which an antibiotic or antimycotic becomes toxic. This is particularly important when using an antimycotic such as Gibco Fungizone reagent or an antibiotic such as tylosin.

The following is a suggested procedure for determining toxicity levels and decontaminating cultures:

1. Dissociate, count, and dilute the cells in antibiotic-free media. Dilute the cells to the concentration used for regular cell passage.
2. Dispense the cell suspension into a multiwell culture plate or several small flasks. Add the antibiotic of choice to each well in a range of concentrations. For example, we suggest the following concentrations for Gibco Fungizone reagent: 0.25, 0.50, 1.0, 2.0, 4.0, and 8.0 µg/mL.
3. Observe the cells daily for signs of toxicity such as sloughing, appearance of vacuoles, decrease in confluency, and rounding.
4. When the toxic antibiotic level has been determined, culture the cells for two to three passages using the antibiotic at a concentration one- to two-fold lower than the toxic concentration.
5. Culture the cells for one passage in antibiotic-free media.
6. Repeat step 4.
7. Culture the cells in antibiotic-free medium for four to six passages to determine if the contamination has been eliminated.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Please view the following page to browse the cell culture antibiotics we offer (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/antibiotics.html).

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

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