Pierce™ Modified Lowry Protein Assay Kit
Pierce™ Modified Lowry Protein Assay Kit
Thermo Scientific™

Pierce™ Modified Lowry Protein Assay Kit

The Pierce Modified Lowry Protein Assay is a stable form of a traditional, two-component, folin phenol- and copper-based reagent systemRead more
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Catalog NumberQuantity
23240530 mL
Catalog number 23240
Price (EUR)
224,65
Online Exclusive
249,00
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Quantity:
530 mL
Request bulk or custom format
Price (EUR)
224,65
Online Exclusive
249,00
Save 24,35 (10%)
Each
Add to cart
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The Pierce Modified Lowry Protein Assay is a stable form of a traditional, two-component, folin phenol- and copper-based reagent system to measure total protein concentration compared to a protein standard.

See all available protein assays ›

Features of Modified Lowry Protein Assay include:
Popular method—widely cited in protein research literature
Colorimetric—measure with a standard spectrophotometer or plate reader at 750 nm
Stability—uses a modified cupric sulfate-tartrate reagent that is stable at room temperature
Assay range—exhibits good linearity in the range 1 to 1500 μg/mL (tested with BSA protein)

The Modified Lowry Protein Assay Kit combines a stabilized formulation of the original Lowry reagents and the essential Folin-Ciocalteu Phenol reagent in a complete kit for accurately determining protein concentration in a variety of samples types. Although newer protein assay methods provide greater speed and convenience, the Lowry method remains a popular, accurate, and useful option for many applications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
AssayLowry Assay
For Use With (Application)Solution-based Detection, Absorbance
For Use With (Equipment)Spectrophotometer, Microplate Reader
Product LinePierce™
Product TypeProtein Quantitation Assay
Quantity530 mL
SpecificityNot Target-Specific
Sufficient For480 Tube Assays or 2400 Microplate Assays
Detection MethodColorimetric
Unit SizeEach
Contents & Storage
Sufficient For: 480 tube assays or 2400 microplate assays

• Modified Lowry Protein Assay Reagent, 480 mL
• 2N Folin-Ciocalteu Phenol Reagent, 50 mL
• Albumin Standard Ampules, 2 mg/mL, 10 x 1 mL

Store at 4°C.
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3213975Certificate of AnalysisJun 11, 202523240
3213975ACertificate of AnalysisJun 11, 202523240
3212114Certificate of AnalysisMay 14, 202523240
AC405702ACertificate of AnalysisMar 19, 202523240
AC405702Certificate of AnalysisMar 19, 202523240
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Safety Data Sheets

Frequently asked questions (FAQs)

The choice of protein assay is dependent on preferences related to assay speed, accuracy and sensitivity, as well as interfering substances in the sample to be assayed. BCA has less protein-to-protein variation, is compatible with most detergents, and has larger working range. Pierce Bradford Plus Protein Assay Kit (Cat. No. 23236) is compatible with reducing sugars, is more sensitive and is faster and easier to use. For a comparison of different protein assays and compatible reagents, see our Tech Tip: Protein Quantitation Assay Compatibility Table (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0068-Protein-assay-compatibility.pdf).

Find additional tips, troubleshooting help, and resources within our Protein Assays and Quantitation Support Center.

The entire kit (3 components) can be stored at 4°C. The storage of individual components is listed below:

  • The Lowry Protein Assay Reagent should be stored at 4°C.
  • The 2N Folin-Ciocalteu Reagent has been tested for one year at both 4°C and room temperature, and shows no difference in performance with both storage conditions.
  • The Albumin Standard Ampules can be stored at 4°C or room temperature. Stability testing for one year at 4°C and at room temperature has also been performed for the Albumin Standards.


Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center

The Pierce Modified Lowry Protein Assay Kit is covered under our general 1-year warranty and is guaranteed to be fully functional for 12 months from the date of shipment, if stored as recommended. Please see section 8.1 of our Terms & Conditions of Sale (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/Terms-and-Conditions-of-Sale.pdf) for more details.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Each of the commonly used total protein assay methods exhibits some degree of varying response toward different proteins. These differences relate to amino acid sequence, pI, structure and the presence of certain side chains or prosthetic groups that can dramatically alter the protein’s color response. Most protein assay methods use BSA or immunoglobulin (IgG) as the standard against which the concentration of protein in the sample is determined. However, if great accuracy is required, prepare the standard curve from a pure sample of the target protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

You can test the tolerance of the assay for your specific buffer formulation. For in-house generated compatibility information, substances were considered compatible at the indicated concentration in the Standard Test Tube Protocol (found in the manual for each protein assay) if the error in protein concentration estimation caused by the presence of the substance was less than or equal to 10%. The substances were tested using WR prepared immediately before each experiment. Blank-corrected 562nm absorbance measurements (for a 1000µg/mL BSA standard + substance) were compared to the net 562nm measurements of the same standard prepared in 0.9% saline.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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