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Gibco™
Sodium Bicarbonate 7.5% solution
Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbonRead more
| Catalog Number | Quantity |
|---|---|
| 25080094 | 100 mL |
Catalog number 25080094
Price (EUR)
17,56
Each
-
Quantity:
100 mL
Price (EUR)
17,56
Each
Sodium Bicarbonate is buffer commonly used for maintaining the pH of cell culture medium in the presence of 4–10% carbon dioxide. In addition to buffering, sodium bicarbonate provides some nutritional benefit, while rarely displaying any cell toxicity. Formulated as 75 g per liter of water, Gibco™ Sodium Bicarbonate, 7.5% Solution is perfect for supplementing dry powder medium during reconstitution. The final Sodium Bicarbonate concentration used in the medium depends on the media formulation and the carbon dioxide concentration used in the incubator.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
Dual-site cGMP Manufacturing and Quality System
Gibco™ Sodium Bicarbonate Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ Sodium Bicarbonate Solution product made in our Scotland facility (25080-060). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
Product Use
For Research Use Only. Not for use in diagnostic procedures.
Dual-site cGMP Manufacturing and Quality System
Gibco™ Sodium Bicarbonate Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical Gibco™ Sodium Bicarbonate Solution product made in our Scotland facility (25080-060). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeMammalian Cells
Concentration0.075
Quantity100 mL
Shelf Life18 Months
Shipping ConditionRoom Temperature
FormLiquid
Product TypeSodium Bicarbonate
SterilitySterile-filtered
pH8.3
Unit SizeEach
Contents & Storage
Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
Shipping conditions: Ambient
Shelf life: 18 months from date of manufacture
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Certificates
Search by lot number or partial lot number
Lot #Certificate TypeDateCatalog Number(s)
3118300Certificate of AnalysisMay 31, 202525080060, 25080094
3118298Certificate of AnalysisMay 23, 202525080060, 25080094
3168534Certificate of AnalysisMay 03, 202525080060, 25080094
3097480Certificate of AnalysisApr 04, 202525080060, 25080094
3011198Certificate of AnalysisJan 31, 202525080060, 25080094
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
If the media formulation contains:
NaHCO3 (g/L) < 1.5, it needs CO2 at 4%;
NaHCO3 (g/L) 1.5 – 2.2, it needs CO2 at 5%;
NaHCO3 (g/L) 2.2 - 3.4, it needs CO2 at 7%;
NaHCO3 (g/L) > 3.5, it needs CO2 at 10% .
*There are some exceptions. Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7 g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. This also depends on the cell type. Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.
Sodium bicarbonate is necessary to control the pH of the solution. To maintain physiological pH, the concentration of the sodium bicarbonate in the medium must be matched with the right level of CO2 in the atmosphere above the medium in the incubator*.
If the sodium bicarbonate is high and the CO2 concentration is low, the pH will become alkaline. This is something that you will see in a bottle of media when it is exposed to air for long periods or when there is a lot of head space in the bottle. The media color will be pinkish or purplish. When this media is put back into a CO2 incubator, the color/pH will change back to the normal orangeish/reddish color or physiological pH.
If the sodium bicarbonate is low and the CO2 is high, the pH will become acidic. This is something you will see when you put medium into a 5% or 10% CO2 incubator.
*There are some exceptions. For instance, Gibco DMEM has always been made according to Dulbecco’s original published formulation, with 3.7g/L sodium bicarbonate. Customers have been using this medium in CO2 incubators ranging from 5-10% CO2 for decades, usually with no trouble maintaining physiological pH. this also depends on the cell type, Once cells are growing, the pH will drop (due to metabolic accumulation of lactic acid).
Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.