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DMEM/F-12, GlutaMAX™ supplement
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many differentRead more
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| Catalog Number | Quantity |
|---|---|
| 31331028 also known as 31331-028 | 500 mL |
10565018 | 500 mL |
| 31331093 also known as 31331-093 | 10 x 500 mL |
Catalog number 31331028
also known as 31331-028
Price (EUR)
24,65
Online Exclusive
28,31Save 3,66 (13%)
Each
Estimated availability date 01-Aug-2025
Quantity:
500 mL
Price (EUR)
24,65
Online Exclusive
28,31Save 3,66 (13%)
Each
DMEM/F-12 (Dulbecco's Modified Eagle Medium/Nutrient Mixture F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts. We offer a variety of DMEM/F-12 modifications for a range of cell culture applications. Find the right formulation using the media selector tool.
This DMEM / F-12 is modified as follows:
| With | Without |
| • GlutaMAX | • HEPES |
| • Phenol Red |
The complete formulation is available.
Using DMEM/F-12
DMEM/F-12 is a 1:1 mixture of DMEM and Ham's F-12. This formulation combines DMEM's high concentrations of glucose, amino acids, and vitamins with F-12's wide variety of components. DMEM/F-12 with GlutaMAX supplement minimizes toxic ammonia build-up and improves cell viability and growth in an easy-to-use format. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.
For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
Concentration1 X
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product TypeDMEM (Dulbecco's Modified Eagle Medium)/F-12
Quantity500 mL
Shelf Life12 Months
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
Sterilization MethodSterile-filtered
With AdditivesGlutaMAX, High Glucose, Phenol Red, Sodium Pyruvate
Without AdditivesNo HEPES
Unit SizeEach
Media Formulations