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Pierce™ ECL Plus Western Blotting Substrate
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-rayRead more
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| Catalog Number | DimensionsLxWxH |
|---|---|
| 32132 | |
| 32134 | |
| 32132X3 |
Catalog number 32132
Price (EUR)
324,65
Online Exclusive
360,00Save 35,35 (10%)
Each
-
Price (EUR)
324,65
Online Exclusive
360,00Save 35,35 (10%)
Each
Thermo Scientific Pierce ECL Plus Substrate is an acridan-based chemiluminescent and chemifluorescent HRP substrate for western blot detection using X-ray film or CCD- or laser-based imagers. This product is sold as Pierce ECL 2 Substrate (Cat. #PI80196X3) through Fisher Scientific and other channels.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
Features of ECL Plus Substrate:
• Easy to use—can be substituted for the discontinued GE ECL Plus Substrate without any re-optimization
• Higher sensitivity—detect targets down to the low-picogram level
• Longer signal duration—sustained light output for as long as 5 hours
• More imaging options—X-ray, CCD or laser-based imagers
• More affordable—high quality and performance at a lower price than other suppliers' similar substrates
Pierce ECL Plus Substrate generates acridinium esters when it reacts with HRP. As these ester intermediates react with peroxide, they produce a strong and sustained chemiluminescent signal that can be captured by X-ray film CCD imaging. The substrate also produces a robust fluorescent signal that can be captured with fluorescence imagers. Pierce ECL Plus Substrate enables the detection of low-picogram amounts of target protein on nitrocellulose or PVDF membrane when probed with appropriate primary and secondary antibody concentrations. Its broad dynamic range, high sensitivity and long-lasting signal make Pierce ECL Plus Substrate an excellent choice for western blot analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
ConjugateHRP
DescriptionPierce ECL Plus Western Blotting Substrate
Kit ContentsDetection Reagent A, 100 mL, Detection Reagent B, 2.5 mL
SensitivityLow picogram to mid-femtogram
Sufficient For10 mini-gel blots, 1000 cm2 of membrane
Detection MethodChemiluminescence, Fluorescence
For Use With (Application)Protein Assay and Analysis
FormLiquid
Membrane CompatibilityNitrocellulose, PVDF
Product LinePierce™
Product TypeSubstrate
Quantity100 mL Kit
Unit SizeEach
Contents & Storage
2°C to 8°C
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Figures
Obtain picogram detection and broad dynamic range using ECL Plus Substrate
Thermo Scientific HRP substrate characteristics
ECL Plus Substrate delivers high performance and a long-lasting signal using three methods of detection
Obtain excellent detection of target proteins with ECL Plus Substrate
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
AA403713ACertificate of AnalysisMay 13, 202532132
AD406099Certificate of AnalysisApr 23, 202532132
AB405125Certificate of AnalysisMar 07, 202532132
ZL403714ACertificate of AnalysisJan 31, 202532134
ZL403714Certificate of AnalysisJan 31, 202532134
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
SuperSignal West Dura Extended Duration Substrate is about 10-fold more sensitive than GE Healthcare ECL Substrate or Thermo Scientific ECL Substrate (Cat. No. 32106). SuperSignal West Dura Extended Duration Substrate is also significantly more sensitive than available chemiluminescent substrates for alkaline phosphatase. Please also see Tech Tip: Guide to Enzyme Substrates for Western Blotting (https://assets.thermofisher.com/TFS-Assets/LSG/Application-Notes/TR0032-Substrates-Western.pdf).
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.
Consider transferring to a different membrane or using a different detection method. We have observed increased sensitivity when using PVDF membranes in place of nitrocellulose. On PVDF membranes, using as little as 1 µg of total rat brain protein, PKC can be detected with alkaline phosphatase-mediated chromogenic detection in some cases using affinity-purified antibodies at a concentration of 0.5 µg/mL. Detection sensitivity can also be increased by using chemiluminescent detection, especially when using a SuperSignal West enhanced chemiluminescence subtrate (https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-assays-analysis/western-blotting/detect-proteins-western-blot/western-blot-detection-reagents/detection-technologies-western-blotting/chemiluminescent-western-blot-detection/supersignal-chemiluminescent-substrates.html) such as SuperSignal West Pico PLUS, SuperSignal West Dura, or SuperSignal West Femto. The secondary antibody should be used as recommend by the manufacturer and optimized as needed.
Find additional tips, troubleshooting help, and resources within our Protein Electrophoresis and Western Blotting Support Center.