DMEM, high glucose
DMEM, high glucose
Gibco™

DMEM, high glucose

DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells.Read more
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Catalog NumberQuantity
41965062
also known as 41965-062
10 x 500 mL
41965039
also known as 41965-039
500 mL
41965047
also known as 41965-047
1000 mL
41965120
also known as 41965-120
6 x 1000 mL
Catalog number 41965062
also known as 41965-062
Price (EUR)
157,65
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207,00
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10 x 500 mL
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Price (EUR)
157,65
Online Exclusive
207,00
Save 49,35 (24%)
Each
Add to cart
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DMEM (Dulbecco's Modified Eagle Medium) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. Life Technologies offers a variety of DMEM modifications for a range of cell culture applications. Find the right formulation using the media selector tool.

This DMEM is modified as follows:
WithWithout
• High Glucose• Sodium Pyruvate
• L-glutamine• HEPES
• Phenol Red


The complete formulation is available.

Using DMEM
DMEM is unique from other media as it contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Product use
For human ex vivo tissue and cell culture processing applications. CAUTION: When used as a medical device, Federal law restricts this device to sale by or on the order of a physician. Customers using Gibco™ DMEM in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).

For Research Use or Further Manufacturing. Not for diagnostic use or direct administration into humans or animals.
Specifications
ClassificationAnimal Origin-free
Concentration1 X
FormLiquid
Product TypeDulbecco's Modified Eagle Medium (DMEM)
Serum LevelStandard Serum Supplementation
Shelf Life12 Months
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Product LineGibco™
Quantity10 x 500 mL
Shipping ConditionRoom Temperature
Osmolality320 - 360 mOsm/kg
pH7.0 to 7.4
Culture EnvironmentCO2
Culture TypeMammalian Cell Culture
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
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