Blasticidin S HCl (10 mg/mL)
Blasticidin S HCl (10 mg/mL)
Gibco™

Blasticidin S HCl (10 mg/mL)

Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis inRead more
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A111390220 mL
A111390310 x 1 mL
Catalog number A1113902
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1.193,65
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1.310,00
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20 mL
Price (EUR)
1.193,65
Online Exclusive
1.310,00
Save 116,35 (9%)
Each
Add to cart
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Blasticidin S is a peptidyl nucleoside antibiotic isolated from Streptomyces griseochromogenes. It is a potent inhibitor of protein synthesis in in bacteria and eukaryotes, while also active against fungi, nematodes, and tumor cells. Blasticidin S acts by blocking hydrolysis of peptidyl-tRNA induced by release factors and inhibits peptide bond formation. It is used as a selection agent in both mammalian cells and bacterial cells. The recommended working concentration ranges from 1 to 30 μg/mL depending on the cell line and 25–100 μg/mL for bacterial selection. Cell death occurs rapidly, and blasticidin-resistant stable mammalian cell lines can be generated in less than one week.

Resistance to blasticidin S is conferred by BSR and BSD, isolated from Bacillus cereus K55-S and Aspergillus terreus respectively. The BSR resistance gene encodes blasticidin S deaminase, which catalyzes the conversion of blasticidin S to deaminohydroxyblasticidin S. Deaminohydroxyblasticidin S is a biologically inactive derivative of blasticidin S and does not interact with or inhibit prokaryotic or eukaryotic ribosomes. The BSD resistance gene also encodes a blasticidin S deaminase, which catalyzes a similar reaction to the BSR deaminase. For bacterial selection purposes, the salt content of the LB medium must remain low (less than 90 mM) and the pH should not exceed 7.0 to maintain the activity of basticidin S. A kill curve is recommended in order to determine the minimum effective blasticidin S concentration to kill non-resistant cells.

Applications
View detailed protocols on blasticidin selection in mammalian cells, E. coli, and yeast.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Cell TypeEukaryotic Cells, Prokaryotic Cells
Concentration10 mg/mL
Culture TypeMammalian Cell Culture, Insect Cell Culture
Product LineGibco™
Quantity20 mL
Shelf Life9 Months
FormLiquid
Product TypeAntibiotic
SterilitySterile-filtered
With AdditivesHEPES
Unit SizeEach
Contents & Storage
Storage conditions: -5°C to -20°C (protect from light)
Shipping conditions: Dry ice
Shelf life: 9 months from date of manufacture
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Frequently asked questions (FAQs)

Which of your antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for stable selection in mammalian cells?

All of our antibiotics (Geneticin, Zeocin, Hygromycin B, Blasticidin, and Puromycin) can be used together for making multiple stable cell lines. However, kill curves will need to be performed for each combination of antibiotics since sensitivity to a given antibiotic tends to increase when combined with other antibiotics.

What are the recommended concentrations of antibiotics to use for selection in prokaryotes and eukaryotes?

For best results, optimal concentrations for selection should be determined empirically in each unique experiment through dose response curves. However, to get a general idea of concentrations that have worked for individual cell types, please click on the following url: http://www.thermofisher.com/us/en/home/life-science/cell-culture/transfection/selection.html or type in “Selection Antibiotics” into our main search on www.thermofisher.com.

What is the mode of action on the following antibiotics: Blasticidin, Geneticin (G418), Hygromycin, and Zeocin?

Blasticidin: Nucleoside Inhibits protein synthesis in prokaryotic and eukaryotic cells by interfering with peptidyl transfer reaction of protein synthesis, causing early termination of translation.

Geneticin (G418): Aminoglycoside Blocks protein synthesis in mammalian cells by interfering with ribosomal function.

Hygromycin: Aminocyclitol Inhibits protein synthesis by disrupting translocation and promoting mistranslation.

Zeocin: Intercalates with DNA and cleaves it.

What is the optimal pH of low salt LB for LB + blasticidin plates?

We recommend a pH of 7 or less and half the normal amount of NaCl in your LB media or plates.

See the following paper for details on optimal conditions: Yamaguchi et al (1965) Inhibition of Protein Synthesis by Blasticidin S. Journal of Biochemistry (Tokyo) Volume 57: pp 667-677.

How long can Blasticidin be stored at 4 degrees C after thawing? Does the unused portion have to be discarded after thawing?

Blasticidin is stable for 6 months when stored at 4 degrees C. Discard remaining material after this time.

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Lot #Certificate TypeDateCatalog Number(s)
1023500Certificate of AnalysisJun 07, 2025A1113903, A1113902
1023498Certificate of AnalysisMay 04, 2025A1113903, A1113902
1023494Certificate of AnalysisApr 25, 2025A1113903, A1113902
1016808Certificate of AnalysisMar 28, 2025A1113903, A1113902
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Citations & References (12)

Citations & References
Abstract
A Scalable, Multiplexed Assay for Decoding GPCR-Ligand Interactions with RNA Sequencing.
Authors:Jones EM, Jajoo R, Cancilla D, Lubock NB, Wang J, Satyadi M, Cheung R, de March C, Bloom JS, Matsunami H, Kosuri S
Journal:Cell Syst
PubMed ID:30904378
'G protein-coupled receptors (GPCRs) are central to how mammalian cells sense and respond to chemicals. Mammalian olfactory receptors (ORs), the largest family of GPCRs, mediate the sense of smell through activation by small molecules, though for most bonafide ligands, they have not been identified. Here, we introduce a platform to ... More
Prioritization of cancer therapeutic targets using CRISPR-Cas9 screens.
Authors:Behan FM, Iorio F, Picco G, Gonçalves E, Beaver CM, Migliardi G, Santos R, Rao Y, Sassi F, Pinnelli M, Ansari R, Harper S, Jackson DA, McRae R, Pooley R, Wilkinson P, van der Meer D, Dow D, Buser-Doepner C, Bertotti A, Trusolino L, Stronach EA, Saez-Rodriguez J, Yusa K, Garnett MJ
Journal:Nature
PubMed ID:30971826
'Functional genomics approaches can overcome limitations-such as the lack of identification of robust targets and poor clinical efficacy-that hamper cancer drug development. Here we performed genome-scale CRISPR-Cas9 screens in 324 human cancer cell lines from 30 cancer types and developed a data-driven framework to prioritize candidates for cancer therapeutics. We integrated cell ... More
ZFP30 promotes adipogenesis through the KAP1-mediated activation of a retrotransposon-derived Pparg2 enhancer.
Authors:Chen W, Schwalie PC, Pankevich EV, Gubelmann C, Raghav SK, Dainese R, Cassano M, Imbeault M, Jang SM, Russeil J, Delessa T, Duc J, Trono D, Wolfrum C, Deplancke B
Journal:Nat Commun
PubMed ID:31000713
'Krüppel-associated box zinc finger proteins (KZFPs) constitute the largest family of mammalian transcription factors, but most remain completely uncharacterized. While initially proposed to primarily repress transposable elements, recent reports have revealed that KFZPs contribute to a wide variety of other biological processes. Using murine and human in vitro and in ... More
A Brain Penetrant Mutant IDH1 Inhibitor Provides In Vivo Survival Benefit.
Authors:Kopinja J, Sevilla RS, Levitan D, Dai D, Vanko A, Spooner E, Ware C, Forget R, Hu K, Kral A, Spacciapoli P, Kennan R, Jayaraman L, Pucci V, Perera S, Zhang W, Fischer C, Lam MH
Journal:Sci Rep
PubMed ID:29062039
'Mutations in IDH1 are highly prevalent in human glioma. First line treatment is radiotherapy, which many patients often forego to avoid treatment-associated morbidities. The high prevalence of IDH1 mutations in glioma highlights the need for brain-penetrant IDH1 mutant-selective inhibitors as an alternative therapeutic option. Here, we have explored the utility ... More
Genome-wide mapping reveals that deoxyuridine is enriched in the human centromeric DNA.
Authors:Shu X, Liu M, Lu Z, Zhu C, Meng H, Huang S, Zhang X, Yi C
Journal:Nat Chem Biol
PubMed ID:29785056
'Uracil in DNA can be generated by cytosine deamination or dUMP misincorporation; however, its distribution in the human genome is poorly understood. Here we present a selective labeling and pull-down technology for genome-wide uracil profiling and identify thousands of uracil peaks in three different human cell lines. Surprisingly, uracil is ... More
12 total citations

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