Amplex™ Red Phospholipase D Assay Kit
Amplex™ Red Phospholipase D Assay Kit
Invitrogen™

Amplex™ Red Phospholipase D Assay Kit

The Amplex™ Red Phospholipase D Assay Kit provides a sensitive and simplemethod to detect Phospholipase D (PLD) activity using aRead more
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Catalog number A12219
Price (EUR)/ 500 assays
650,00
-
Add to cart
Price (EUR)/ 500 assays
650,00
Add to cart
Amplex™ Red Phospholipase D Assay Kit
Catalog numberA12219
Price (EUR)/ 500 assays
650,00
-
Add to cart
The Amplex™ Red Phospholipase D Assay Kit provides a sensitive and simplemethod to detect Phospholipase D (PLD) activity using a fluorescence microplate reader or fluorometer.

See our complete line of Fluorescence Microplate assays.

• Detect phospholipase D activity levels as low as 10 U/mL
• Format allows for multiple time point measurements
• Designed for minimal autofluorescence interference

PLD activity is monitored indirectly using 10-acetyl-3,7-dihydroxyphenoxazine (Amplex™ Red reagent), a sensitive fluorogenic probe for hydrogen peroxide. PLD converts the phosphatidylcholine (lecithin) to choline, which is then oxidized by choline oxidase to betaine and hydrogen peroxide. In the presence of horseradish peroxidase, huydrogen peroxide reacts with the Amplex™ Red reagent in a 1:1 stoichiometric ratio to generate the highly fluorescent product resorufin.

Because resorufin has absorption and fluorescence emission maxima of approximately 571 nm and 585 nm, respectively, there is little interference from autofluorescence in most biological samples.

Use Amplex™ Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex™ Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex™ UltraRed Reagent (Cat. No. A36006), a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex™ Red/UltraRed Stop Reagent (Cat. No. A33855). The Amplex™ Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours. Custom assay design and packaging are also available.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence Intensity
For Use With (Application)Phospholipase Assay
For Use With (Equipment)Fluorescence Microplate Reader
Product TypePhospholipase Assay
Product LineAmplex™
Quantity500 assays
Shipping ConditionRoom Temperature
Substrate PropertiesLipid-Based Substrate, Chemical Substrate
Substrate TypePhospholipase Substrate
Target EnzymePhospholipase
Unit Size500 assays
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.