ExpiCHO™ Expression Medium
Share
ExpiCHO™ Expression Medium
Gibco™

ExpiCHO™ Expression Medium

A GMP-manufactured, chemically-defined, serum-free, protein-free, animal origin–free medium specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells.
Have Questions?
Change viewbuttonViewtableView
Catalog NumberQuantity
A291000420 L
A29100011000 mL
A29100026 x 1 L
A291000310 L
Catalog number A2910004
Price (EUR)
8.355,65
Online Exclusive
9.830,00
Save 1.474,35 (15%)
Each
-
Add to cart
Quantity:
20 L
Price (EUR)
8.355,65
Online Exclusive
9.830,00
Save 1.474,35 (15%)
Each
Add to cart

ExpiCHO Expression Medium is specifically designed for high-density growth and transfection of suspension-adapted Chinese hamster ovary (CHO) cells. This medium is GMP-manufactured, chemically-defined, serum-free, protein-free, and animal origin–free. It is a core component of the ExpiCHO Expression System Kit (Cat. No. A29133) for scalable transient protein production. To achieve the highest levels of transient protein expression, the medium is matched to a culture feed, ExpiCHO Feed, also included in the system kit and transfection kits.

With ExpiCHO Expression Medium you can:

  • Grow suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability
  • Achieve high transfection efficiencies of >90% when coupled with ExpiFectamine CHO transfection reagent
  • Transiently transfect suspension CHO cells at a density of 6 x 106 cells per mL
  • Achieve yields up to 3 g per L of recombinant protein of high-density transiently transfected cultures by sustained, high-level expression
  • Grow and transfect CHO cells at volumes of <1 mL up to 5 L in culture formats from multiwell plates up to 5 L shake flask

ExpiCHO Expression Medium is ready to use. It is formulated with GlutaMAX-I reagent and does not require supplementation. It does not contain phenol red. The chemically-defined, animal origin free formulation results in high reproducibility and lot-to-lot reliability. ExpiCHO Expression Medium is not recommended for adherent cell cultures.

Specifications
Cell LineExpiCHO-S™, CHO-S, SFM Adapted
FormatUniversal Bag
Product LineExpiCHO™
Product TypeCHO Expression Medium
Quantity20 L
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free, Chemically-defined, Protein-free, Serum-free
Culture TypeSuspension Cell Culture
FormLiquid
Serum LevelSerum-free
With AdditivesGlutaMAX
Without AdditivesNo Phenol Red
Unit SizeEach
Contents & Storage
Storage conditions: in the dark at 2-8°C
Shipping conditions: ambient
Shelf life: 12 months from date of manufacture
Please enter sku to load default questions

Frequently asked questions (FAQs)

My ExpiCHO Expression Medium (Cat. No. A2910002) was accidentally frozen. Can I still use it?

This product should be stored at 2-8 degrees C and should not be frozen. The biggest issue with accidentally freezing this product is its solubility. If this product was accidentally frozen, we recommend placing it in a 2-8 degree environment and allowing it to slowly thaw overnight. If the product is fully thawed and shows no signs of precipitation, then it should still be usable, but we cannot guarantee effectiveness.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

When subculturing ExpiCHO the day before a transfection to 3-4 x 10E6/mL, would you recommend pelleting the cells and resuspending them in completely fresh media, or simply replacing some of the media to split them down to the desired cell density?

Before transfecting ExpiCHO cells, there is no need to change out the media during the subculturing. This would become expensive and the conditioned media is not necessary.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

For how many passages would you recommend using ExpiCHO cells before replacing them with new cells that were frozen at earlier passages?

For optimal performance with respect to protein production, we recommend thawing ExpiCHO cells and letting them grow for 2-3 passages prior to transfection. Overall, we do not recommend using the cells beyond ˜20 passages.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How many days should I wait before harvesting my protein with the ExpiCHO system?

Optimal time to harvest protein will depend on the specific properties of the protein being expressed and the protocol chosen. Typical harvest times to reach maximum titers for the various protocols are as follows:
- Standard Protocol: 8-10 days post-transfection
- High Titer Protocol: 10-12 days post-transfection
- Max Titer Protocol: 12-14 days post-transfection

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

I didn't add the enhancer with the ExpiCHO system. Will I still get good protein expression?

The Enhancer is designed to work with the ExpiCHO system for maximal expression. If the enhancer is not added, there will be a greater than 50% loss in expression.

Find additional tips, troubleshooting help, and resources within our Protein Expression Support Center.

View all ExpiCHO™ Expression Medium FAQs

Documents & Downloads

Certificates

Search by lot number or partial lot number
Search
Request a Certificate

Safety Data Sheets

Share catalog number, name or link