Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. ItRead more
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Catalog Number
Quantity
B0008
250 mL
B0007
10 mL
Catalog number B0008
Price (EUR)/ 250 mL
145,65
Online exclusive
156.00
Save 10,35 (7%)
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Add to cart
Quantity:
250 mL
Price (EUR)/ 250 mL
145,65
Online exclusive
156.00
Save 10,35 (7%)
Add to cart
4X Bolt™ LDS Sample Buffer
Catalog numberB0008
Price (EUR)/ 250 mL
145,65
Online exclusive
156.00
Save 10,35 (7%)
-
Add to cart
Bolt LDS Sample Buffer (4X) is used to prepare protein samples for denaturing gel electrophoresis with Bis-Tris Plus gels. It contains lithium dodecyl sulfate, pH 8.4, which allows for maximum activity of the reducing agent.
Bolt LDS Sample Buffer contains Coomassie G250 and Phenol Red as tracking dyes instead of bromophenol blue. Coomassie G250 gives a sharp dye front with both MES and MOPS SDS running buffers and migrates much closer to the moving ion front than bromophenol blue. Bromophenol blue runs more slowly than some peptides with MES SDS Running Buffer. This ensures that small peptides do not run off the gels.
To use: Heat the sample in a 1X dilution (reduced or non-reduced) at 70°C for 10 minutes for optimal results.
Note: Bolt LDS Sample Buffer should be brought to room temperature (25°C) prior to use. It is a highly viscous and concentrated solution containing twice the amount of LDS compared to the amount of SDS in typical sample buffers. Bolt LDS Sample Buffer also contains a higher concentration glycerol.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
BufferSample Loading Buffers
Concentration4X
For Use With (Equipment)Bolt Mini Gel Tank
Gel TypeBis-Tris Plus
Product TypeLDS Sample Buffer
Gel CompatibilityBolt™ Bis-Tris Plus Gels
Product LineBolt™
Quantity250 mL
Unit Size250 mL
Contents & Storage
Sample Buffer (4X) containing lithium dodecyl sulfate (LDS) at pH 8.5 with SERVA Blue G250 and phenol red.