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Application
FIGURE: 1 / 2
FMO3 Antibody (CF810419) in WB
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FMO3 (RC206506, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-FMO3. (1. Positive lysates LY416335 (100 µg) and LC416335 (20 µg) can be purchased separately from OriGene.
Product Details
CF810419
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
OTI1G4
Immunogen
Human recombinant protein fragment corresponding to amino acids 1-291 of human FMO3 produced in E.coli.
Conjugate
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 8% trehalose
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
For reconstitution, we recommend adding 100 µL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiments, we strongly recommend performing another round of desalting. (Zeba Spin Desalting Columns, 7KMWCO, 0.5 mL, Product # 89882)
Target Information
The mammalian flavin-containing monooxygenases (FMO; EC 1.14.13.8) represent a multigene family whose gene products are localized in the endoplasmic reticulum of many tissues. These enzymes catalyze the NADPH-dependent oxidative metabolism of many drugs, pesticides, and other foreign compounds. Their substrates are soft nucleophiles with an electron-rich center, typically a nitrogen, sulfur or phosphorus-containing functional group, as the site for oxidative attack by the enzyme.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Dimethylaniline monooxygenase [N-oxide-forming] 3; Dimethylaniline oxidase 3; Flavin-containing monooxygenase 3; FMO 3; FMO form 2; FMO II; Hepatic flavin-containing monooxygenase 3; hepatic flavin-containing monooxygenase-3; MGC34400; Trimethylamine monooxygenase
Gene Aliases: AW111792; dJ127D3.1; FMO3; FMOII; TMAU
UniProt ID: (Human) P31513, (Mouse) P97501
Entrez Gene ID: (Human) 2328, (Mouse) 14262
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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