Chargeswitch™ Pro Filter Collection Tube
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Invitrogen™

Chargeswitch™ Pro Filter Collection Tube

The Chargeswitch™-Pro Filter Collection Tube is a 50 mL conical tube that will accommodate the centrifuge protocol of Chargeswitch™ ProRead more
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Catalog NumberQuantity
CS3200025 x 50 mL Tubes
Catalog number CS32000
Price (EUR)
28,35
Each
Add to cart
Quantity:
25 x 50 mL Tubes
Price (EUR)
28,35
Each
Add to cart

The Chargeswitch™-Pro Filter Collection Tube is a 50 mL conical tube that will accommodate the centrifuge protocol of Chargeswitch™ Pro Filter Plasmid Midi and Maxi kits.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Quantity25 x 50 mL Tubes
Shipping ConditionRoom Temperature
Tube TypeCollection Tube
Unit SizeEach
Contents & Storage
25 x 50 mL conical tubes. Store at room temperature

Frequently asked questions (FAQs)

I used water to elute my plasmid instead of the Elution Buffer with my ChargeSwitch kit, but saw very low plasmid yield. Why is this?

We strongly recommend using the Elution Buffer provided in the kit, and do not recommend elution with water. If you need to elute in any other buffer, be sure to use a buffer of pH 8.5-9.0 for efficient DNA elution.

What is the difference between the ChargeSwitch Pro kit and the Charge-Switch Pro filter kit?

The filter kit is based upon a dual nested column design, which facilitates the fastest and simplest protocol for obtaining high quality plasmid (less than 10 EU/µg DNA). Modified wash buffer 1 removes more endotoxin.

What is the typical yield when using the ChargeSwitch Pro Filter Plasmid Mini or Midi Kits?

The typical yield is 25 µg from a 5 mL culture for the ChargeSwitch Pro mini kit, 200 µg from a 25 mL culture for the midi kit, and 800 µg from a 100 mL culture from the maxi kit. The plasmid size range for the kits is typically 3-9 kb.

How does ChargeSwitch technology work?

Based on a patented switchable chemistry, ChargeSwitch purification utilizes the charge on a solid surface for nucleic acid capture. At lower pH, the surface chemistry holds a positive charge and attracts nucleic acid. At higher pH, the surface chemistry is neutral and any bound nucleic acid is released.

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