Using the EnzChek Phosphatase Assay Kit continuous monitoring of phosphatase activity, even at neutral or acidic pH, can be achieved. The kit contains Molecular Probes' patented 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) substrate (D6567). DiFMUP is about 100 times more sensitive than 4-methylumbelliferyl phosphate (MUP) for the detection of prostatic acid phosphatase at pH 5.5. The kit is perfect for the continuous assay of prostatic acid phosphatase, protein phosphatase 1 or almost any other phosphatase that can be assayed with nonprotein-based substrates such as MUP or 4-nitrophenyl phosphate (PNPP). Each kit contains a sufficient amount of substrate for ∼1000 assays, using a reaction volume of 100 μL per assay. Fluorescence (excitation/emission maxima ∼358/455 nm) can be measured in a fluorescence microplate reader or a standard fluorometer.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Color
Blue
Detection Method
Fluorescent
For Use With (Application)
Phosphatase Assay
For Use With (Equipment)
Microplate Reader
Product Type
Phosphatase Assay
Dye Type
DiFMU
Emission
360⁄460
Product Line
EnzChek™
Shipping Condition
Room Temperature
Substrate
DiFMUP
Substrate Properties
Chemical Substrate
Substrate Type
Phosphatase Substrate
Target Enzyme
Phosphatases
Contents & Storage
Store in freezer -5°C to -30°C and protect from light.