Maxima Reverse Transcriptase (200 U/μL)
Maxima Reverse Transcriptase (200 U/μL)
Thermo Scientific™

Maxima Reverse Transcriptase (200 U/μL)

Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependentRead more
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Catalog NumberQuantity
EP07412,000 Units
EP074210,000 Units
EP07434 x 10,000 Units
Catalog number EP0741
Price (EUR)
49,05
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Online exclusive
Ends: 15-Aug-2025
81,75
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Quantity:
2,000 Units
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Price (EUR)
49,05
Special offer
Online exclusive
Ends: 15-Aug-2025
81,75
Save 32,70 (40%)
Each
Add to cart
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Thermo Scientific Maxima Reverse Transcriptase (RT) was developed through in vitro evolution of M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity as well as RNase H activity. The engineered enzyme features dramatically improved thermostability and robustness, and increased synthesis rates compared to wild type M-MuLV RT.

Maxima Reverse Transcriptase is capable of reproducible cDNA synthesis from a wide range of input total RNA amounts (from 1 pg to 5 μg) at elevated temperatures (50–65°C), which makes this enzyme an ideal tool for two step RT-qPCR (see supporting data).

Due to its high thermostability, Maxima enzyme maintains full activity during the entire reverse transcription reaction, generates the highest yields of cDNA, and is able to synthesize even very long RNA transcripts up to 20 kb. The reaction temperature can be increased up to 65°C for efficient transcription of RNA regions with a high secondary structure or to improve specificity using gene specific primers. Due to its increased synthesis rate, the reverse transcriptase reaction can be completed in 15 to 30 minutes

Features of Maxima Reverse Transcriptase include:
• High yields of full-length cDNA up to 20 kb
• Active up to 65°C
• Thermostable—90% active after incubation at 50°C for 60 minutes
• High sensitivity allows reproducible cDNA synthesis from a wide range of total RNA quantities (1 pg to 5 μg)
• Efficient formula for complete cDNA synthesis in 15 to 30 minutes
• Incorporates modified nucleotides

Applications
• Two step RT-PCR
• Two step RT-qPCR
• First strand cDNA synthesis
• Construction of full length cDNA libraries
• DNA labeling
• Primer extension

Also available
Maxima Reverse Transcriptase
Maxima First Strand cDNA Synthesis Kit for RT-qPCR
Maxima First Strand cDNA Synthesis Kit for RT-qPCR, with dsDNase

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration200 U/μL
Final Product TypeFirst-Strand cDNA
FormatStand-alone Enzyme
Optimal Reaction Temperature50°C to 55°C
Quantity2,000 Units
Reaction FormatSeparate components
Reagent TypeReverse Transcription
Reverse TranscriptaseMaxima
Ribonuclease H ActivityYes
Shipping ConditionDry Ice
Size (Final Product)Up to 20 kb
Starting MaterialRNA
TechniqueReverse Transcription
GC-Rich PCR PerformanceHigh
Reaction Speed30 min.
Unit SizeEach
Contents & Storage

• Maxima Reverse Transcriptase, 1 x 2,000 units (200 U/μL)
• 5X RT Buffer

Store at –20°C.

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Frequently asked questions (FAQs)

When should I choose regular RevertAid RT or Maxima RT vs. RevertAid H-minus RT or Maxima H-minus RT?

It is generally beneficial to minimize RNase H activity when aiming to produce long transcripts for cDNA cloning. RNase H degrades RNA from RNA-DNA duplexes, which can result in truncated cDNA during reverse transcription of long mRNA. It is also recommended to use RNase H-minus RTs for template-independent addition of C nucleotides. In contrast, reverse transcriptases with intrinsic RNase H activity are often favored in qPCR applications.

Do Thermo Scientific reverse transcriptases (RevertAid RT, RevertAid H-minus RT, Maxima RT, and Maxima H-minus RT) possess terminal deoxynucleotidyl (TdT) activity?

All Thermo Scientific reverse transcriptases possess intrinsic TdT activity although at varying degrees depending upon the reaction conditions. For addition of template-independent C nucleotides (as for SMART and RACE experiments), this specific TdT activity can be induced by Mn2+. We would recommend Maxima H- or RevertAid H- minus RTs for this purpose.

Is it preferable to use elevated temperature in the RT reaction when using Maxima reverse transcriptases?

cDNA synthesis at higher temperatures ensures successful transcription of RNA with high levels of secondary structure, reducing issues of primer access to template. Therefore, we do recommend to use RT enzymes with high thermostability, e.g. Maxima and Maxima H Minus Reverse Transcriptases, which provide higher yields of full-length cDNA, better sensitivity, and successful transcription of GC-rich templates.

What steps should I take while performing first strand cDNA synthesis using low purity template (e.g., inhibitors in RNA sample)?

Trace amounts of reagents used in RNA purification protocols may remain in solution and inhibit first-strand synthesis, e.g., SDS, EDTA, guanidine salts, phosphate, pyrophosphate, polyamines, spermidine. To remove trace contaminants, we recommend re-precipitating the RNA with ethanol and washing the pellet with 75% ethanol, or re-purifying the RNA.

For reverse transcription, how important is the quality of RNA template?

RNA purity and integrity are essential for synthesis and quantification of cDNA. Always assess the integrity of RNA prior to cDNA synthesis. Use freshly prepared RNA. Multiple freeze/thaw cycles of the RNA sample and synthesized cDNA is not recommended. Avoid RNase contamination and discard low quality RNA.

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