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Invitrogen™
FluoSpheres™ Carboxylate-Modified Microspheres
Achieve the brightest fluorescence with Carboxylate-Modified FluoSphere Microspheres, available in different colors and particle sizes.
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| Catalog Number | Diameter (Metric) | Color | Excitation/Emission | Quantity |
|---|---|---|---|---|
| F8789 also known as F-8789 | 0.04 μm | Dark Red | 660/680 nm | 1 mL |
| F8803 | 0.1 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8816 | 1.0 μm | Crimson | 625/645 nm | 2 mL |
| F8823 | 1.0 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8801 | 0.1 μm | Red | 580/605 nm | 10 mL |
| F8811 | 0.2 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8807 | 0.2 μm | Dark Red | 660/680 nm | 2 mL |
| F10720 | 0.04 μm | Yellow-Green, Orange, Red, Dark Red | 505/515, 540/560, 580/605, 660/680 nm | 1 mL/each |
| F20881 | 0.2 μm | Orange | 365/610 nm | 2 mL |
| F8783 also known as F-8783 | 0.02 μm | Dark Red | 660/680 nm | 2 mL |
| F8786 | 0.02 μm | Red | 580/605 nm | 10 mL |
| F8795 also known as F-8795 | 0.04 μm | Yellow-Green | 505/515 nm | 1 mL |
| F8813 | 0.5 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8820 | 1.0 μm | Orange | 540/560 nm | 10 mL |
| F8825 | 2.0 μm | Nile Red | 535/575 nm | 2 mL |
| F8827 | 2.0 μm | Yellow-Green | 505/515 nm | 2 mL |
| F8781 also known as F-8781 | 0.02 μm | Blue | 365/415 nm | 10 mL |
| F8782 also known as F-8782 | 0.02 μm | Crimson | 625/645 nm | 2 mL |
| F8784 | 0.02 μm | Nile Red | 535/575 nm | 10 mL |
| F8787 | 0.02 μm | Yellow-Green | 505/515 nm | 10 mL |
| F8792 also known as F-8792 | 0.04 μm | Orange | 540/560 nm | 1 mL |
| F8793 | 0.04 μm | Red | 580/605 nm | 1 mL |
| F8794 | 0.04 μm | Red-Orange | 565/580 nm | 1 mL |
| F8797 | 0.1 μm | Blue | 350/440 nm | 10 mL |
| F8799 | 0.1 μm | Infrared | 715/755 nm | 1 mL |
| F8800 also known as F-8800 | 0.1 μm | Orange | 540/560 nm | 10 mL |
| F8805 | 0.2 μm | Blue | 365/415 nm | 10 mL |
| F8806 | 0.2 μm | Crimson | 625/645 nm | 2 mL |
| F8809 | 0.2 μm | Orange | 540/560 nm | 10 mL |
| F8810 | 0.2 μm | Red | 580/605 nm | 10 mL |
| F8812 | 0.5 μm | Red | 580/605 nm | 10 mL |
| F8814 | 1.0 μm | Blue | 365/415 nm | 10 mL |
| F8815 | 1.0 μm | Blue | 350/440 nm | 10 mL |
| F8819 | 1.0 μm | Nile Red | 535/575 nm | 10 mL |
| F8821 | 1.0 μm | Red | 580/605 nm | 10 mL |
| F8824 also known as F-8824 | 2.0 μm | Blue | 365/415 nm | 2 mL |
| F8826 | 2.0 μm | Red | 580/605 nm | 2 mL |
Catalog number F8789
also known as F-8789
Price (EUR)
698,00
Each
-
Diameter (Metric):
0.04 μm
Color:
Dark Red
Excitation/Emission:
660/680 nm
Quantity:
1 mL
Price (EUR)
698,00
Each
Easily perform flow cytometry, microscopy, HTS, HCS, immunoassay, and other laboratory applications using our extensive selection of FluoSpheres Carboxylate-Modified Microspheres. FluoSphere beads can be used in passive adsorption or active, covalent coupling of proteins, nucleic acids, and biomolecules for particle capture applications. FluoSphere microspheres are loaded with proprietary fluorescent dyes, making them the brightest microspheres available.
Visualize the brightest fluorescence for laboratoy applications including fluorescence microscopy, flow cytometry, HTS, HCS, and cell tracing with our Carboxylate-Modified FluoSphere Microspheres, which are manufactured from polystyrene microspheres and loaded with different proprietary dyes. Using specialized staining methods enables all of the fluorescent dye molecules to be contained inside each polystyrene microsphere instead of on the bead's surface. This protective environment within the bead shields the dye from detrimental environmental effects, such as photobleaching. Our carboxylate-modified microspheres are coated with a hydrophilic polymer containing multiple carboxylic acids for covalent attachment of ligands. A range of particle sizes is available for different research uses and experiments.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Excitation/Emission660/680 nm
Product LineFLUOSPHERES™
Quantity1 mL
Surface ModificationCarboxylate
ColorDark Red
Diameter (Metric)0.04 μm
For Use With (Application)Fluorescence Microscopy
MaterialPolystyrene
Product TypeCarboxylate-Modified Microsphere
Unit SizeEach
Contents & Storage
Store in refrigerator (2–8°C) and protect from light.
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FluoSpheres® fluorescent microspheres.
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3154551Certificate of AnalysisJun 20, 2025F8783
3223927Certificate of AnalysisJun 20, 2025F8813
3193695Certificate of AnalysisJun 20, 2025F8821
3193696Certificate of AnalysisJun 17, 2025F8810
3154554Certificate of AnalysisMay 04, 2025F8826
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Safety Data Sheets
Frequently asked questions (FAQs)
The CML beads have a high density of carboxyl groups at the surface. The surface layer is quite hydrophilic and at the appropriate pH (pH>5), are charged; due to electrostatic repulsion, this type of surface is 3-dimensional and may be considered analogous to the fuzz on a tennis ball.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The smaller the microspheres, the greater the propensity to aggregate. But the aggregation is not irreversible. Sonicate in a bath sonicator or vortex to disperse, just prior to use. You can also add a small concentration of Tween-20 or Triton X-100 (unless you are using them in a live-cell system).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Use of a bath sonicator is recommended to help break up any aggregated microspheres. The foaming is from Tween-20, which is in the stock solution to help prevent aggregation. It is normal and expected to see bubbles from this. Do not use a probe sonicator, which would cause damage to the microspheres (as well as much more bubbling).
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
The warranty period for FluoSpheres microspheres is 1-year from the date of shipment.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Centrifugation is not an effective way to collect smaller microspheres; many particles remain in the solution even if you can visualize a small pellet. For beads less than 1 µm in diameter, we recommend washing by either:
Cross-flow filtration, as these particles have a very high compression modulus and can withstand high g-forces without risk of harm or dialysis with a 500 kDa MWCO
Note: Microspheres greater than 1 µm in diameter can be centrifuged at 1,300 rpm.
Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.
Citations & References (124)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.
Journal:Biophysical journal
PubMed ID:17114225
Functionalized quantum dots offer several advantages for tracking the motion of individual molecules on the cell surface, including selective binding, precise optical identification of cell surface molecules, and detailed examination of the molecular motion without photobleaching. We have used quantum dots conjugated with integrin antibodies and performed studies to quantitatively
Tuftsin binds neuropilin-1 through a sequence similar to that encoded by exon 8 of vascular endothelial growth factor.
Journal:The Journal of biological chemistry
PubMed ID:16371354
Live imaging of lymphatic development in the zebrafish.
Journal:Nature medicine
PubMed ID:16732279
Cell lines as candidate reference materials for quality control of ERBB2 amplification and expression assays in breast cancer.
Journal:Clin Chem
PubMed ID:19443566
Human epidermal growth factor receptor 2 (HER2) is an important biomarker whose status plays a pivotal role in therapeutic decision-making for breast cancer patients and in determining their clinical outcomes. Ensuring the accuracy and reproducibility of HER2 assays by immunohistochemistry (IHC) and by fluorescence in situ hybridization (FISH) requires a
Morphology and dynamics of clathrin/GGA1-coated carriers budding from the trans-Golgi network.
Journal:Mol Biol Cell
PubMed ID:12686608
'Sorting of transmembrane proteins and their ligands at various compartments of the endocytic and secretory pathways is mediated by selective incorporation into clathrin-coated intermediates. Previous morphological and biochemical studies have shown that these clathrin-coated intermediates consist of spherical vesicles with a diameter of 60-100 nm. Herein, we report the use
124 total citations