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Thermo Scientific™
Genomic DNA Purification Kit
Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from variousRead more
| Catalog Number | Quantity |
|---|---|
| K0512 | 100 Preps |
Catalog number K0512
Price (EUR)
186,00
Each
-
Quantity:
100 Preps
Price (EUR)
186,00
Each
Thermo Scientific Genomic DNA Purification Kit is a simple and rapid system for high quality genomic DNA purification from various sources including: whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues.
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction
Applications
• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol
The kit is based on selective detergent-mediated DNA precipitation from crude lysate. The entire procedure is rapid only 20 to 25 minutes with a typical yield of 2 to 10 μg genomic DNA from 0.2 mL of blood.
High molecular weight genomic DNA purified with the kit is suitable for direct use in all common molecular biology applications: PCR, FastDigest and conventional restriction digestion, cloning, DNA sequencing, and Southern blot analysis.
Highlights
• Efficient—2 to 10 μg of genomic DNA from 0.2 mL of blood
• Fast—procedure takes approximately 20 minutes
• Universal—purifies genomic DNA from various sources
• Flexible—easy to scale up and down
• Safe—does not involve phenol extraction
Applications
• Purification of high-quality genomic DNA from:
• Whole blood or serum
• Cell culture
• Plant tissues
• Mammalian tissues
• Epithelium samples
• Bacteria Includes Lysis Solution Precipitation Solution NaCl Solution Detailed Protocol
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Elution Volume20 to 100 μL
Final Product TypegDNA
For Use With (Application)PCR, Southern blotting, sequencing, nucleic acid labeling, hybridization
High-throughput CompatibilityNot High-throughput Compatible (Manual)
Quantity100 Preps
Sample TypeBacteria, Cells, Plant, Serum, Tissue, Whole Blood, Blood
Shipping ConditionRoom Temperature
Starting Material AmountBacteria: ≤20 mg
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL
Cells: ≤106
Plant: ≤100 mg
Tissue: ≤30 mg
Serum: ≤200 μL
Whole Blood: ≤200 μL
Test Time25 min.
Isolation TechnologyOrganic Extraction
Unit SizeEach
Contents & Storage
• 40 mL Lysis Solution; room temperature
• 8 mL Precipitation Solution (concentrated); room temperature
• 10 mL NaCl Solution; room temperature
• 8 mL Precipitation Solution (concentrated); room temperature
• 10 mL NaCl Solution; room temperature
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