This cell line is available as dividing cells by requesting a quote for K1226 from dividingcells@invitrogen.com

GeneBLAzer™ H2 HEK 293T DA (Division Arrested) cells and H2-CRE-bla HEK 293T cells contain the human Histamine Subtype 2 receptor (H2) stably integrated into the CellSensor™ CRE-bla HEK 293T cell line. CellSensor™ CRE-bla HEK 293T cells (Cat. no.K1112) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE). Division Arrested (DA) cells are available in two configurations: an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates.

DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both H2 HEK 293T DA cells and H2-CRE-bla HEK 293T cells are functionally validated for Z’-factor and EC₅₀ concentrations of Histamine (Figure 1). In addition, H2-CRE-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

Histamine 2 (H2) receptors have been implicated in a number of disorders, including peptic ulcer disease (PUD), gastroesophageal reflux disease (GERD), dyspepsia, and stress ulcer prophylaxis.


Storage:

GeneBLAzer™ H2 HEK 293T DA Cells and H2-CRE-bla HEK 293T Cells are shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use.


Contents:
GeneBLAzer™ H2 HEK 293T DA Assay Kit
Each system contains sufficient division arrested (DA) cells & substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).Includes:
•H2 HEK 293T DA cells
•LiveBLAzer™-FRET B/G Loading Kit, 70μg
•Solution D, 1ml
•2 protocols
•Certificate of Analysis
GeneBLAzer™ H2 HEK 293T DA cells
Each system contains sufficient division arrested (DA) cells to assay 10 x 384-well plates (LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•H2 HEK 293T DA cells
•1 protocol
•Certificate of Analysis
GeneBLAzer™ H2-CRE-bla HEK 293T cells
(LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol).
Includes:
•H2-CRE-bla HEK 293T cells
•1 protocol
•Certificate of Analysis