InVision™ His-Tag In-Gel Stain
InVision™ His-Tag In-Gel Stain
Invitrogen™

InVision™ His-Tag In-Gel Stain

InVision His-Tag In-Gel Stain is a ready-to-use, fast, sensitive, and highly specific fluorescent stain for visualizing His-tagged fusion protein bandsRead more
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Catalog NumberQuantity
LC6030500 mL
Catalog number LC6030
Price (EUR)
848,00
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Quantity:
500 mL
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Price (EUR)
848,00
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InVision His-Tag In-Gel Stain is a ready-to-use, fast, sensitive, and highly specific fluorescent stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.

Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours

Compare all His-tag stains ›

Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Product LineInVision™
Product TypeHis Tag Proteins In-Gel Stain
Quantity500 mL
Shelf Life6 Months
Target MoleculeProteins (His-Tagged)
Label or DyeInVision His-Tag Stain
Unit SizeEach
Contents & Storage
InVision™ His-tag In-gel Stain is supplied as a 1X ready-to-use staining reagent. Store at room temperature. Guaranteed stable for 6 months when properly stored.

Frequently asked questions (FAQs)

I used the InVision His-Tag In-Gel Stain to stain my His-Tagged fusion protein and used the BenchMark His-tagged protein standard as a positive control. I am seeing some additional bands with the BenchMark His-tagged Protein Standard. What went wrong?

This is likely due to overexposure - performing a longer exposure to detect low expression levels of the desired protein may result in staining of minor contaminants in the BenchMark His-tagged Protein Standard. Load less BenchMark His-tagged protein Standard or perform a short exposure to visualize and image the standard and then perform a longer exposure to visualize and image proteins expressed at low levels.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the InVision His-Tag In-Gel Stain and am getting high, uneven background. Can you please offer some tips?

Here are possible causes and solutions:

- Missed washing steps. Be sure to wash the gel twice with 20 mM phosphate buffer. If the background is high, perform a third water wash step for 10 minutes.
- Poor water quality. Use ultrapure water (>18 megohm/cm) for washing and preparing phosphate buffer.
- Protein overloaded. Decrease the protein concentration or lower the sample volume.
- Dirty imaging platform. Always clean the imaging system with a paper towel prior to imaging the gel to minimize any background fluorescence.
- Non-specific bands. Highly basic proteins and divalent metal binding proteins such as carbonic anhydrase (30 kDa), SlyD (21 kDa), and phosphorylase B (97 kDa) may cross-react with the stain producing non-specific bands.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the InVision His-Tag In-Gel Stain and am getting very weak staining. Can you please offer some tips?

Here are possible causes and solutions:

- Inadequate staining. Use appropriate staining protocol based on the gel type. Use BenchMark His-tagged Protein Standard as a positive control to verify staining reagents and protocol. Avoid excessive washing of the gel.
- The gel is not visualized or imaged properly. Be sure to visualize the gel using a UV transilluminator equipped with a camera or a laser-based scanner using the correct filters (see manual for details). A Polaroid camera is not recommended. Make sure the aperture on the camera is open wide to allow enough light entry and that the camera is connected to imaging software that allows contrast adjustment for viewing the best image. Visualize the gel immediately after completing the washing steps. Storing the gel in phosphate buffer decreases the signal intensity.
- Low protein load or expression level. Check total protein content of the gel by staining the gel with a total protein stain (check page 13 of the manual). Load at least 1 pmole of the His-tagged fusion protein for detection. Make sure the His-tag is in-frame and the protein is expressed properly.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Can I stain E-PAGE gels with InVision His-Tag In-Gel Stain?

E-PAGE gels are thicker than standard mini-gels and result in too much background when stained with InVision His-Tag In-Gel Stain. To obtain better staining sensitivity, we recommend transferring proteins of E-PAGE gels onto a nitrocellulose membrane and then staining the blot with the InVision His-tag In-gel Stain as described on page 14 in the manual.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I have stained my His-tagged fusion protein with InVision His-Tag In-Gel Stain. Can I perform western blotting of the stained protein?

To perform western blotting after InVision His-Tag In-Gel staining of His-tagged fusion proteins:

- Record a permanent image of the gel after staining of His-tagged fusion proteins.
- Equilibrate the gel in 1X SDS Running Buffer for 1 hour.
- Perform western blotting and immunodetection using a method of choice.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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