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Neon™ NxT Electroporation System 100-μL Kit with 1-Channel Tubes
The Neon NxT Electroporation System 100-μL Kit is designed specifically for use with the Neon NxT Electroporation System.
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| Catalog Number | No. of Reactions |
|---|---|
| N10025 | 25 x 2 Reactions |
| N10096 | 96 x 2 Reactions |
Catalog number N10025
Price (EUR)
715,65
Online Exclusive
822,00Save 106,35 (13%)
Each
No. of Reactions:
25 x 2 Reactions
Price (EUR)
715,65
Online Exclusive
822,00Save 106,35 (13%)
Each
The Neon NxT Electroporation System 100-μL Kit with 1-Channel Tubes is designed specifically for use with the Neon NxT Electroporation System (Cat. Nos. NEON1S, NEON1SK, NEON18S, NEON18SK). Use this kit for transfection volumes of 100 μL, containing 5 x 105–2 x 106 adherent cells or 1 x 106–5 x 106 suspension cells. Cells that have been transfected using the included 100-μL Neon NxT tips are then ready to be washed and plated into a multi-well culture dish.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)Transfection
For Use With (Equipment)Neon™ NxT Electroporation System
No. of Reactions25 x 2 Reactions
Product TypePipette Tip
Quantity25 x 2 reactions
Volume (Metric) Sample100 μL
Cell TypeStem Cells, Primary Cells, Hard-to-Transfect Cells
FormatElectroporation Pipette Tip
Transfection TechniqueElectroporation
Unit SizeEach
Contents & Storage
• 10 mL Resuspension R Buffer; store at 4°C
• 10 mL Resuspension T Buffer; store at 4°C
• 10 mL Resuspension Genome Editing Buffer; store at 4°C
• 50 mL Electrolytic E100 Buffer; store at 4°C
• 25 reaction tips
• 8 electroporation 1-channel tubes
• 10 mL Resuspension T Buffer; store at 4°C
• 10 mL Resuspension Genome Editing Buffer; store at 4°C
• 50 mL Electrolytic E100 Buffer; store at 4°C
• 25 reaction tips
• 8 electroporation 1-channel tubes
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Safety Data Sheets
SDSFrequently asked questions (FAQs)
We recommend warming the buffers to room temperature before use.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
We recommend storing Neon NxT buffers in the original containers at 4 degrees C.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Neon NxT buffers should be stored at 4 degrees C. Temperature fluctuations and storage at room temperature have been shown to increase the chances for precipitate formation. Therefore, the desired amount of buffer for an experiment should be removed from the buffer bottle and the bottle should be immediately returned to 4 degrees C.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
For best results with the Neon Nxt Electoporation System, the cells should be healthy (not stressed) and actively dividing so that the nuclear membrane integrity is already weakened for payload needing to enter the nucleus. Consider “starving” the cells of serum or other essential nutrients.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.
Arcing could be caused by high voltage or pulse length settings, high salt or contaminants in the DNA sample, incorrect cell density, and air bubbles formed during mixing of cell samples. Mix samples gently to avoid air bubble formation and pipette samples in a slow, smooth motion to avoid air uptake.
Find additional tips, troubleshooting help, and resources within our Transfection Support Center.