SYBR™ Safe DNA Gel Stain
SYBR™ Safe DNA Gel Stain
Invitrogen™

SYBR™ Safe DNA Gel Stain

Green features
SYBR™ Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR™Read more
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Catalog NumberQuantity
S33102Promo Image400 μL
Catalog number S33102
Price (EUR)
88,40
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Ends: 15-Aug-2025
136,00
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Quantity:
400 μL
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Price (EUR)
88,40
Special offer
Online exclusive
Ends: 15-Aug-2025
136,00
Save 47,60 (35%)
Each
Add to cart
SYBR™ Safe DNA Gel Stain is a highly sensitive stain for visualization of DNA in agarose or acrylamide gels. SYBR™ Safe stain is specifically formulated to be a less hazardous alternative to ethidium bromide that can utilize either blue light or UV excitation.

• Reduce your exposure to highly mutagenic ethidium bromide and harmful UV light
• Increase your sensitivity by reducing nonspecific background fluorescence
• Use in place of ethidium bromide for all staining applications, including RNA staining

A better DNA stain
SYBR™ Safe DNA Gel Stain is a better nucleic acid staining reagent for all of your molecular biology needs. Not only is SYBR™ Safe DNA Gel Stain better for you and the environment, it’s better for your sample and your institution. Read more as to why SYBR™ Safe DNA gel stain is your better option.

Safer than ethidium bromide
SYBR™ Safe DNA Gel Stain has been specifically formulated and evaluated in a battery of toxicity tests with results indicating so that it is less mutagenic and safer for you to work with than ethidium bromide. An independent laboratory showed reduced mutagenicity of SYBR™ Safe stain compared to ethidium bromide using the Ames test (see figure). You can further decrease your exposure risk by using visible blue-light illumination with SYBR™ Safe stain rather than UV illumination. This is especially valuable when performing exposure-intensive protocols like cutting bands out of gels.

Excellent sensitivity
SYBR™ Safe stain offers excellent sensitivity in nucleic acid visualization and documentation applications with either UV excitation or blue-light excitation. When bound to nucleic acids, the green-fluorescent SYBR™ Safe stain has fluorescence excitation maxima at ∼280 and ∼502 nm, and an emission maximum at ∼530 nm (see figure). Plus, when used with blue light illumination, SYBR™ Safe stain has less background fluorescence than ethidium bromide–stained gels illuminated with UV light.

Easy to use
SYBR™ Safe stain is supplied as a 10,000X concentrate in DMSO which can be used just like a solution of ethidium bromide. SYBR™ Safe stain can be mixed into an agarose gel for staining during electrophoresis or the gel can be incubated in a solution of SYBR™ Safe stain following electrophoresis. SYBR™ Safe stain can be stored at room temperature in its original packaging to avoid excessive light exposure. We also offer SYBR™ Safe E-Gel pre-cast agarose gels for the ultimate in convenience.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection LocationIn-Gel Detection
Detection MethodFluorescence
Green FeaturesLess hazardous
Product TypeDNA Gel Stain
Quantity400 μL
Shelf Life6 months from date of purchase
Shipping ConditionRoom Temperature
Target MoleculeDNA
Label or DyeSYBR Safe
Unit SizeEach
Contents & Storage
• Supplied as a 10,000X concentrate in DMSO

Store at room temperature in the original container.

Frequently asked questions (FAQs)

Why do I sometimes see speckles in my gel when using SYBR Safe DNA Gel Stain?

Many whitening agents used in clothing, as well as some fungi and bacteria, fluoresce at the same wavelengths as SYBR Safe DNA gel stain. These contaminants within or on the surface of the gel may produce this speckling.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the pH range of SYBR dyes?

The SYBR dyes are useful only over a narrow range of pH, from about 7 to 8. Outside this range, the fluorescent signal diminishes rapidly.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Which direction does the SYBR Safe dye run during electrophoresis?

Similarly to ethidium bromide, SYBR Safe DNA Gel Stain runs in the opposite direction of the migrating DNA. This has no practical effect on the use of gels cast with SYBR Safe DNA Gel Stain, as only the very bottom of the gel will have a lower concentration of stain. This effect can be partially counteracted by staining the gel with SYBR Safe DNA Gel Stain after electrophoresis. Solutions of dye should not be added to the running buffer as this can cause breakdown of the dye at the electrodes and release toxic volatile compounds into the air.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Does ethanol precipitation remove the SYBR Safe dye?

SYBR Safe DNA Gel Stain is easily removed from nucleic acids by ethanol precipitation.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Can I reuse SYBR Safe DNA Gel Stain for a second gel?

We strongly discourage the reuse of SYBR Safe DNA Gel Stain, as this practice significantly lowers sensitivity.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

Fluorescence spectra

Fluorescence spectra

Documents & Downloads

Certificates

Safety Data Sheets

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