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The pLenti4⁄V5-DEST™ Gateway™ Vector is a Gateway™-adapted ViraPower™ lentiviral expression vector for lentiviral-based expression of a target gene in dividing and non-dividing mammalian cells. The vector has the CMV promoter for driving constitutive expression of the target gene and the Zeocin™ selection marker for stable selection in mammalian cells.
Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells
Key Features
• Flexible and versatile Gateway™ recombination cloning technology
• Constitutive high expression with CMV promoter
• Zeocin™ selection marker for stable selection
• C terminal V5 tag for quick detection
Kit includes
• pLenti4⁄V5-DEST™ Gateway™ Vector
• One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)
Related SKUs
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V49910)
• pLenti6⁄V5-DEST™ Gateway™ Vector (V49610)
• pLenti6⁄V5 Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit (K495000)
• ViraPower™ Lentiviral Gateway™ Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K5330-00)
For research use only. Not intended for any therapeutic or diagnostic use.
Advantages
• Lentivirus based expression of a target gene in dividing and non-dividing mammalian cells
Key Features
• Flexible and versatile Gateway™ recombination cloning technology
• Constitutive high expression with CMV promoter
• Zeocin™ selection marker for stable selection
• C terminal V5 tag for quick detection
Kit includes
• pLenti4⁄V5-DEST™ Gateway™ Vector
• One Shot™ Stbl3™ Chemically Competent E. coli (C7373-03)
Related SKUs
• pLenti6⁄UbC⁄V5-DEST™ Gateway™ Vector (V49910)
• pLenti6⁄V5-DEST™ Gateway™ Vector (V49610)
• pLenti6⁄V5 Directional TOPO™ Cloning Kit (K4955-10)
• ViraPower™ Lentiviral Directional TOPO™ Expression Kit (K495000)
• ViraPower™ Lentiviral Gateway™ Expression Kit (K4960-00)
• ViraPower™ HiPerform™ Lentiviral Gateway™ Expression Kit (K5330-00)
For research use only. Not intended for any therapeutic or diagnostic use.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Lentiviral
Promoter
CMV
Product Type
Lentiviral Expression Vector
Selection Agent (Eukaryotic)
Zeocin™
Protein Tag
V5 Epitope Tag
Cloning Method
Gateway
Vector
pDEST, pLenti
Product Line
Gateway™, ViraPower™
For Use With (Application)
Viral Expression
Contents & Storage
ViraPower™ Lentiviral Gateway™ Vectors store at–20°C includes:
pLenti4⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)
pLenti4⁄V5-GW⁄lacZ control: 10 μg (20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)
One Shot™ Stbl3™ Chemically Competent E. coli store at –80°C
pLenti4⁄V5-DEST™: 6 μg (40 μl of 150 ng⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)
pLenti4⁄V5-GW⁄lacZ control: 10 μg (20 μl of 0.5 μg⁄μl vector in 10 mM Tris-HCl, 1mM EDTA, pH 8.0)
One Shot™ Stbl3™ Chemically Competent E. coli store at –80°C
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