The InsectSelect™ BSD Kit features the pIB/V5-His vector for stable expression of your gene of interest in a variety of common insect cells. This vector has several features that facilitate expression, analysis, and detection of recombinant protein in insect cells:
• The OpIE2 promoter for constitutive expression • The Blasticidin resistance gene for rapid selection of stably transfected cells in two weeks • C-terminal V5 epitope and polyhistidine (6xHis) sequence for detection with Invitrogen's Anti-V5 Antibody and rapid purification with nickel-chelating resin
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Promoter
OplE2
Product Type
Insect Cell Expression Vector
Protein Tag Position (to your gene)
C-terminal
Protein Tag
His Tag (6x), V5 Epitope Tag
Cloning Method
Restriction Enzyme/MCS
Vector
pIB
Product Line
InsectSelect™
Contents & Storage
The InsectSelect pIB/V5-His Vector Kit includes 20 μg each of supercoiled lyophilized pIB/V5-His and pIB/V5-CAT vectors, and 2 μg lyophilized OplE2 Forward and Reverse sequencing primers.
Store all reagent at -20°C. All reagents are guaranteed stable for 6 months when properly stored.