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McCoy's 5A (Modified) Medium
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cellRead more
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| Catalog Number | Quantity |
|---|---|
| 26600023 also known as 26600-023 | 500 mL |
| 26600080 also known as 26600-080 | 10 x 500 mL |
Catalog number 26600023
also known as 26600-023
Price (EUR)
40,97
Each
Quantity:
500 mL
Price (EUR)
40,97
Each
McCoy's 5A (modified) is a general purpose medium that supports the propagation of many types of primary cells, established cell lines, and explants from biopsy tissues. This medium will support the growth of primary mammalian cells derived from normal bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
This McCoy's 5A is modified as follows:
| With | Without |
| • High Glucose | • Sodium Pyruvate |
| • L-glutamine | • HEPES |
| • Bacto-peptone | |
| • Phenol Red |
The complete formulation is available.
Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.
Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.
Specifications
Cell TypeMammalian Cells
Concentration1 X
Culture EnvironmentCO2
Manufacturing QualitycGMP-compliant under the ISO 13485 standard
Osmolality280 - 320 mOsm/kg
Product LineGibco™
Product TypeMcCoy's 5A Medium
Quantity500 mL
Shelf Life12 Months
Shipping ConditionRoom Temperature
ClassificationAnimal Origin-free
Culture TypeMammalian Cell Culture
FormLiquid
SterilitySterile-filtered
With AdditivesHigh Glucose, Glutamine, Phenol Red, Sodium Bicarbonate
Without AdditivesNo HEPES, No Sodium Pyruvate
pH6.9 to 7.1
Unit SizeEach
Contents & Storage
Storage conditions: 2-8° C. Protect from light.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
Shipping conditions: Ambient.
Shelf life: 12 months from date of manufacture.
