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SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits
SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits
Thermo Scientific™

SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits

Obtain fast, simple, highly reproducible, and sensitive streptavidin immunocapture and trypsin protein digestion in a single well for your biomarker studies.

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Catalog NumberIncludes
60110-103SMART Digest IA Kit, Av magnetic with SOLAμ SPE and collection plate
60110-101SMART Digest IA Kit, Streptavidin (Av) non-magnetic
60110-102SMART Digest IA Kit, Av non-magnetic with SOLAμ SPE and collection plate
60110-104SMART Digest IA Kit, Av magnetic
60110-104-NW-10PKBulk Magnetic beads with immobilized streptavidin and trypsin for capture and digestion and SMART Digest buffer
Catalog number 60110-103
Price (EUR)
1.742,23
Each
Add to cart
Includes:
SMART Digest IA Kit, Av magnetic with SOLAμ SPE and collection plate
Price (EUR)
1.742,23
Each
Add to cart

Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The kit purifies various target biomolecules via the formation of strong streptavidin-biotin bond.

Solving the Challenges of Low-level Quantitation in Complex Matrices

The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.

As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.

Immunocapture and Protein Digestion in a Single Well

These IA kits deliver a process that is:

  • Fast
  • Simple
  • Highly reproducible
  • Sensitive

Unique Design Reduces Workflow to Hours

In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.

Specifications
DescriptionSMART Digest IA Kit, Av magnetic with SOLAμ SPE and collection plate
For Use With (Application)Peptide Quantitation
IncludesSMART Digest IA Kit, Av magnetic with SOLAμ SPE and collection plate
CaptureStreptavidin
CleavageC-terminal side of lysine and arginine amino acids
EnzymeTrypsin
FormatSMART Digest Resin, Magnetic
Product LineSMART Digest
TypeImmunocapture + Protein Digestion combined on a single bead
Unit SizeEach
Putting Peptides on the Map

Putting Peptides on the Map

How do advances in digestion, separation, mass detection & data interpretation remove roadblocks in protein characterization?

Download eBook ›

Faster and More Sensitive Protein Characterization and Quantitation

Faster and More Sensitive Protein Characterization and Quantitation

How can characterization and quantitation of bio-therapeutics can be made easier, faster and more reproducible?

Download eBook ›

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