Contents & Storage
Bac-to-Bac™ HBM TOPO™ Cloning Kit contains: (For details see the manual)
• Vector kit for 20 topo cloning reactions
• pFastBac⁄HBM TOPO™ Vector containing the C-terminal TEV cleavage sit and His-Tag.
• pFastBac⁄CT-Gus Control Plasmid (Control expression vector)
• Other reagents supplied: 10x PCR, dNTP Mix, Salt Solution, Sterile Water, Control PCR template, Polyhedrin Forward Primer, SV40 pA Reverse Primer
• Kit containing competent cells (20 reactions):
• One Shot™ Mach1-T1R Chemically Competent E. coli
Vector kit: store at -20°C
One Shot™ Mach1-T1R Chemically Competent E. coli: store at -80°C