Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
POROS CaptureSelect CH1-XL Affinity HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect affinity ligands. CaptureSelect CH1-XL Affinity HPLC columns recognize the CH1 domain of human IgG antibodies, enabling quantitation and small-scale purification of all IgG subclasses (IgG1, IgG2, IgG3, and IgG4), as well as Fab fragments and fusion proteins containing the intact CH1 domain, independent of light-chain iso-type and source material.Analytical groups supporting biologic drug development require rapid, sensitive, and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates. It is critical for biopharmaceutical manufacturers to be able to monitor bioreactor product titers to determine when to harvest or abort a reactor cycle.
Features of POROS CaptureSelect CH1-XL Affinity HPLC columns include:
High speed with high specificity
POROS high performance chromatography allows for the quantitation of product from clarified harvest or partially purified intermediates—typically in under three minutes. Moreover, only minimal sample preparation (0.2 μm filtration) is required, and the eluted product is highly purified and suitable for further analysis, including mass spectrometry.
Automation
POROS Affinity HPLC columns are for use with HPLC systems, which allows the automation of chromatography methods, data collection, and data analysis. In addition, the immobilized affinity ligand enables one method and buffer system to be used across a class of molecules, streamlining analytical approaches.
Multiple cycles
Proper sample preparation, adherence to column operating instructions, and the use of an optimized column cleaning regimen results in strong re-use performance. POROS Affinity HPLC columns can be cycled hundreds of times for cost-effective and reproducible quantitation or purification.
Main characteristics