Biotin XX Tyramide SuperBoost™ Kit, Goat anti-Mouse IgG
Biotin XX Tyramide SuperBoost™ Kit, Goat anti-Mouse IgG
Invitrogen™

Biotin XX Tyramide SuperBoost™ Kit, Goat anti-Mouse IgG

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC),Read more
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Catalog NumberQuantity
B40911150 Slides
Catalog number B40911
Price (EUR)
596,00
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150 Slides
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Price (EUR)
596,00
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SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features biotin XX tyramide with poly-HRP-conjugated goat anti-mouse IgG. For detection and additional signal amplification, conjugated streptavidin is used to detect specifically deposited biotin XX .

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Product TypeTyramide Kit
Quantity150 Slides
Shipping ConditionApproved for shipment at Room Temperature or on Wet Ice
ConjugateBiotin-XX
Product LineSuperBoost™
Unit SizeEach
Contents & Storage
1 kit sufficient for 150 microscope slides (18 x 18 mm), containing: Blocking buffer (1X), 22.5 mL
  • Poly-HRP-conjugated goat anti-mouse secondary antibody (1X), 22.5 mL
  • Biotin-XX tyramide reagent
  • Hydrogen peroxid
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    Frequently asked questions (FAQs)

    With a SuperBoost tyramide kit, I got excessive and non-specific labeling. What can I do to limit background and acquire a more localized labeling?

    To limit background, we recommend performing a pre-blocking step with 3% H2O2 for 60 mins to inactivate endogenous peroxidases. To limit the localization of labeling, we recommend optimizing the final concentration of the primary and secondary antibodies and the dye-tyramide. You may also limit the incubation time of the dye-tyramide on the sample.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    Is it possible to perform dual TSA labeling with SuperBoost tyramide kits?

    Yes. This involves the sequential application of the antibodies and the tyramides with a HRP-quenching step between antibodies using H2O2.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

    How are SuperBoost tyramide kits different from the original TSA labeling kits?

    The SuperBoost tyramide kits utilize poly-HRP labeled antibodies. This provides a greater number of horseradish peroxidase (HRP) molecules per antibody. The original kits used antibodies and streptavidin that were directly conjugated with HRP and thus, limited the number per antibody or streptavidin.

    Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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