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Invitrogen™
Click-iT™ Plus EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 594 dye
Click-iT Plus EdU Cell Proliferation imaging kits have been optimized for fluorescence microscopy applications and are a superior alternative toRead more
| Catalog Number | Quantity |
|---|---|
| C10639 | 1 kit |
Catalog number C10639
Price (EUR)
818,00
Each
Quantity:
1 kit
Price (EUR)
818,00
Each
Click-iT Plus EdU Cell Proliferation imaging kits have been optimized for fluorescence microscopy applications and are a superior alternative to traditional proliferation assays. In this assay the modified thymidine analogue EdU (5-ethynyl-2'-deoxyuridine, a nucleoside analog of thymidine) is efficiently incorporated into newly synthesized DNA and fluorescently labeled with a bright, photostable Alexa Fluor™ dye in a fast, highly-specific, mild click reaction.
Find more tools for image-based detection of proliferating cells >
• Simple—works the first time, every time, in less time than traditional methods
• Efficient—no denaturation steps or harsh treatment required
• Content-rich results—improved preservation of cell morphology, antigen structure, GFP fluorescent signal, and DNA integrity
• Consistent—not dependent on variable antibody lots for detection
The kit contains all of the components needed to label and detect the incorporated EdU as well as perform cell cycle analysis on samples from adherent cells. For cell cycle analysis, the kit includes a blue fluorescent Hoechst 33342 dye. The kit contains sufficient reagents for labeling 50 18x18 coverslips using 500 μL of reaction buffer per test.
Avoid the Harsh Treatments Associated with BrdU Method
Measuring changes to cell proliferation is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate method of doing this is by directly measuring DNA synthesis. The traditional method utilizes the nucleoside analog BrdU (5-bromo-2'-deoxyuridine, a nucleoside analog of thymidine), which is incorporated into newly transcribed DNA. After incorporation the samples are treated with harsh methods (HCl, heat, or enzymes) to denature the DNA and expose the BrdU molecules to detection by anti-BrdU antibodies. However, the BrdU method to measure cell proliferation is time consuming and difficult to perform consistently. The harsh treatments necessary for this method can adversely affect sample integrity, cell morphology, image quality, and the ability to multiplex.
The Click-iT™ Plus EdU assay measures the rate of new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. Detection is achieved through a catalyzed 'click' reaction that is completed typically within 30 minutes. The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, producing low backgrounds and high detection sensitivities. Because of the mild reaction conditions the Click-iT™ Plus assays can accurately determine cell proliferation while preserving cell morphology, DNA integrity, antigen binding sites, and the fluorescent signal from GFP. Preservation of DNA integrity allows for DNA staining, including staining with dyes used for cell cycle analysis.
Find more tools for image-based detection of proliferating cells >
• Simple—works the first time, every time, in less time than traditional methods
• Efficient—no denaturation steps or harsh treatment required
• Content-rich results—improved preservation of cell morphology, antigen structure, GFP fluorescent signal, and DNA integrity
• Consistent—not dependent on variable antibody lots for detection
The kit contains all of the components needed to label and detect the incorporated EdU as well as perform cell cycle analysis on samples from adherent cells. For cell cycle analysis, the kit includes a blue fluorescent Hoechst 33342 dye. The kit contains sufficient reagents for labeling 50 18x18 coverslips using 500 μL of reaction buffer per test.
Avoid the Harsh Treatments Associated with BrdU Method
Measuring changes to cell proliferation is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The most accurate method of doing this is by directly measuring DNA synthesis. The traditional method utilizes the nucleoside analog BrdU (5-bromo-2'-deoxyuridine, a nucleoside analog of thymidine), which is incorporated into newly transcribed DNA. After incorporation the samples are treated with harsh methods (HCl, heat, or enzymes) to denature the DNA and expose the BrdU molecules to detection by anti-BrdU antibodies. However, the BrdU method to measure cell proliferation is time consuming and difficult to perform consistently. The harsh treatments necessary for this method can adversely affect sample integrity, cell morphology, image quality, and the ability to multiplex.
The Click-iT™ Plus EdU assay measures the rate of new DNA synthesis based on incorporation of the nucleoside analog EdU into DNA. Detection is achieved through a catalyzed 'click' reaction that is completed typically within 30 minutes. The click reaction uses bioorthogonal (biologically unique) moieties to fluorescently label proliferating cells, producing low backgrounds and high detection sensitivities. Because of the mild reaction conditions the Click-iT™ Plus assays can accurately determine cell proliferation while preserving cell morphology, DNA integrity, antigen binding sites, and the fluorescent signal from GFP. Preservation of DNA integrity allows for DNA staining, including staining with dyes used for cell cycle analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeAlexa Fluor™ 594
FormatVial(s)
Green FeaturesLess hazardous
Quantity1 kit
For Use With (Application)Cell Viability, Proliferation and Function
For Use With (Equipment)Fluorescence Microscope
Product LineClick-iT
Product TypeCell Proliferation Kit
Unit SizeEach
Contents & Storage
Contains EdU (5-ethynyl-2' -deoxyuridine), Alexa Fluor™ dye-picolyl azide, anhydrous dimethylsulfoxide (DMSO), Click-iT™ EdU reaction buffer, Copper Protectant, Click-iT™ EdU buffer additive, and Hoechst 33342.Store at 2°C to 8°C Dessicate and protect from light.
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Frequently asked questions (FAQs)
Figures
Comparison of Click-iT EdU and Click-iT Plus EdU
Click-iT® Plus EdU Alexa Fluor® 594 Imaging Kit vs. BrdU
GFP and proliferation signal detected in HeLa cells
Click-iT® Plus EdU Alexa Fluor® 594 proliferation assay in action
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Certificates
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Lot #Certificate TypeDateCatalog Number(s)
3143114Certificate of AnalysisApr 03, 2025C10639
2980640Certificate of AnalysisOct 07, 2024C10639
2738315Certificate of AnalysisNov 19, 2023C10639
2559157Certificate of AnalysisJan 30, 2023C10639
2471817Certificate of AnalysisMar 17, 2022C10639
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Product Information
Limited Use Label Licenses (LULL)
'
Limited Use Label License No. 402: Nucleic acid labeling and detection
Notice to Purchaser: If end-user or third party is interested in a commercial license, it should contact Harvard's Office of Technology Development, 1350 Massachusetts Avenue, Holyoke Center, Suite 727, Cambridge, MA 02138, (617) 495-3067.
Protocols
Citations & References (12)
Search citations by name, author, journal title or abstract text
Citations & References
Abstract
Silencing of STRN4 suppresses the malignant characteristics of cancer cells.
Journal:
PubMed ID:25250919
The striatin family of proteins, comprising STRN, STRN3 and STRN4, are multidomain-containing proteins that associate with additional proteins to form a large protein complex. We previously reported that STRN4 directly associated with protein kinases, such as MINK1, TNIK and MAP4K4, which are associated with tumor suppression or tumor progression. However,
Primary cilia control hedgehog signaling during muscle differentiation and are deregulated in rhabdomyosarcoma.
Journal:
PubMed ID:24927541
The primary cilium acts as a cellular antenna, transducing diverse signaling pathways, and recent evidence suggests that primary cilia are important in development and cancer. However, a role for cilia in normal muscle development and rhabdomyosarcoma (RMS) has not been explored. Here we implicate primary cilia in proliferation, hedgehog (Hh)
Heterogeneous Responses to Mechanical Force of Prostate Cancer Cells Inducing Different Metastasis Patterns.
Journal:Adv Sci (Weinh)
PubMed ID:32775149
Transcriptome dynamics of hippocampal neurogenesis in macaques across the lifespan and aged humans.
Journal:Cell Res
PubMed ID:35750757
The Primate-Specific Gene TMEM14B Marks Outer Radial Glia Cells and Promotes Cortical Expansion and Folding.
Journal:Cell Stem Cell
PubMed ID:29033352
12 total citations