Carboxy-DCFDA can passively diffuse into cells and is colorless and nonfluorescent until the acetate groups are cleaved by intracellular esterases to yield the fluorescent fluorophore, 5-(and-6)-carboxy-2',7' -dichlorofluorescein. With a pK_a of 4.8, this dye can be used as an acidic pH sensor.

To make a concentrated stock solution from 1–10 mM, dissolve in either high quality anhydrous DMSO, DMF, or 100% ethanol. Once prepared, use the stock solutions within a short time period.

Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›