Phusion Flash High-Fidelity PCR Master Mix
Phusion Flash High-Fidelity PCR Master Mix
Thermo Scientific™

Phusion Flash High-Fidelity PCR Master Mix

Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time.
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Catalog NumberNo. of Reactions
F548S100 Reactions
F548L500 Reactions
Catalog number F548S
Price (EUR)/ Each
97,25
-
Add to cart
No. of Reactions:
100 Reactions
Request bulk or custom format
Price (EUR)/ Each
97,25
Add to cart
Phusion Flash High-Fidelity PCR Master Mix
Catalog numberF548S
Price (EUR)/ Each
97,25
-
Add to cart
Thermo Scientific Phusion Flash PCR Master Mix was developed to save valuable laboratory time. Ready-to-use 2X master mix preserves the fidelity and the yield in reactions when using very short PCR protocols. The unique master mix composition permits usage of short cycling protocols with both low and high complexity DNA templates (15 seconds per kilobase or less). The master mix utilizes Phusion Flash II DNA Polymerase, a modified proofreading DNA polymerase derived from Phusion Hot Start II High-Fidelity DNA Polymerase.

Features

  • Extreme speed with extension times of 15 s/kb or less
  • Hot start modification allows 'zero-time reactivation'
  • Accurate proofreading DNA polymerase with a fidelity of 25X Taq
  • High yields in reduced cycling time
  • Minimized pipetting steps

Applications

  • Fast PCR
  • High-fidelity PCR
  • Difficult (GC-rich) templates
  • Template generation for sequencing Multiplex PCR
  • Long-range PCR
  • Cloning & mutagenesis
  • Microarray

Notes

  • Annealing rules for Phusion DNA Polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePhusion Flash II DNA Polymerase
Reaction SpeedFast
Product TypeHigh-Fidelity PCR Master Mix
Quantity100 reactions
Shipping ConditionDry Ice
For Use With (Application)Hot-start PCR, High-fidelity PCR
Concentration2X
Fidelity (vs. Taq)25X
Hot StartBuilt-In Hot Start
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Size (Final Product)20 kb or less
ColorColorless
Unit SizeEach
Contents & Storage
Phusion Flash High-Fidelity PCR Master Mix (1 mL)

Store at -20°C.