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ProPac™ HIC-10 HPLC Columns
Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns.
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| Catalog Number | Particle Size | Diameter (Metric) | Length (Metric) |
|---|---|---|---|
| SP6869 | 5 μm | 22 mm | 250 mm |
| 063653 | 5 μm | 2.1 mm | 100 mm |
| 063655 | 5 μm | 4.6 mm | 100 mm |
| 063665 | 5 μm | 7.8 mm | 75 mm |
| 074197 | 5 μm | 4.6 mm | 250 mm |
| SP6800 | 5 μm | 10 mm | 150 mm |
Catalog number SP6869
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Particle Size:
5 μm
Diameter (Metric):
22 mm
Length (Metric):
250 mm
Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multi-functional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in HIC.
High-efficiency Columns for the Separation of Large Biomolecules
- High protein loading capacity for protein purification applications
- Wide range of analytical and preparative applications, including the separation of bovine serum proteins, snake venom proteins, and monoclonal antibodies (Mabs)
- Based on 5μm ultra-high purity spherical silica gel particles with 300Å pores
Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.
Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.
Specifications
Column FormatPreparative Column, Standard
Diameter (Metric)22 mm
Length (Metric)250 mm
Max. Pressure3500 psi (240 bar)
Particle Size5 μm
pH2.5 to 7.5
Pore Size300 Å
Surface Area100 m²/g
Temperature40°C
Stationary PhaseAmide/Ethyl
Product LineProPac
Packing MaterialSpherical, Fully Porous, Ultrapure Silica
Column TypeHydrophobic Interaction
PhaseHydrophobic Interaction
MaterialStainless Steel
Unit SizeEach