Inhibidor de la ribonucleasa recombinante RNaseOUT™
Inhibidor de la ribonucleasa recombinante RNaseOUT™
Invitrogen™

Inhibidor de la ribonucleasa recombinante RNaseOUT™

El inhibidor de ribonucleasa recombinante RNaseOUT™ es un potente inhibidor no competitivo de ribonucleasas de tipo pancreático como ARNasa A.Más información
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Número de catálogoCantidad
107770195000 units
Número de catálogo 10777019
Precio (EUR)
323,00
Special offer
Online exclusive
termina: 21-Jul-2025
348,00
Ahorro 25,00 (7%)
Each
En Stock
Añadir al carro de la compra
Cantidad:
5000 units
Recurring order eligible. Learn more »
Pedido a granel o personalizado
Precio (EUR)
323,00
Special offer
Online exclusive
termina: 21-Jul-2025
348,00
Ahorro 25,00 (7%)
Each
Añadir al carro de la compra
Ask our AI about this Product
El inhibidor de ribonucleasa recombinante RNaseOUT™ es un potente inhibidor no competitivo de ribonucleasas de tipo pancreático como ARNasa A. Se utiliza para evitar la degradación del ARN en una serie de aplicaciones. El inhibidor de ribonucleasa recombinante RNaseOUT™ es una proteína acídica con un peso molecular de ∼52 kDa. RNaseOUT™ inhibe la ARNasa A, RNasa B y RNasa C.

Aplicaciones
Síntesis de la ADNc, RT-PCR y transcripción y translación in vitro

Fuente
Purificado por cromatografía de afinidad a partir de la expresión de un gen porcino clonado de E. coli

Pruebas de rendimiento y calidad
Pureza SDS-PAGE, ensayos de endodesoxiribonucleasa, concentración de proteínas, actividad específica, rendimiento evaluado mediante RT-PCR

Definición de la unidad
Una unidad inhibe 5 ng de ARNasa A en un 50 % mediante el uso de citidina 2´, 3´ monofosfato cíclico (cCMP) como sustrato

Condiciones de reacción de la unidad
100 mM de tris-acetato (pH 6,5), 1 mM de EDTA, 0,2 mM de cCMP, 2 µg de ARNasa A en 1 ml de 0 a 10 minutos a 25 °C
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Concentración100 mm
Línea de productosRNaseOUT™
Tiempo de purificación10 min
Cantidad5000 units
Condiciones de envíoHielo seco
Unit SizeEach
Contenido y almacenamiento
Almacenar a -20 °C. Evite exponer el producto a cambios frecuentes de temperatura. El inhibidores de ribonucleasa RNaseOUT™ requiere 1 mm de DTT para mantener la actividad.

El producto tiene una garantía de 6 meses a partir de la fecha de compra, a menos que se indique lo contrario en la documentación del producto.
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Preguntas frecuentes

Which components of the SuperScript III First Strand Synthesis System for RT-PCR are available for purchase separately?

The following components are available as stand-alone items:

- Superscript III Reverse Transcriptase (Cat. Nos. 18080093, 18080044, 18080085)
- Oligo (dT)20 Primer (Cat. No. 18418020)
- Random hexamers (Cat. No. 48190011)
- 10 mM dNTP Mix (Cat. Nos. 18427013, 18427088)
- RNAseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)
- E. coli RNAse H (Cat. Nos. 18021014, 18021071)

What does RNaseOUT Recombinant RNase Inhibitor do?

The inhibitor acts as a safeguard against degradation of target RNA due to ribonuclease contamination of the RNA preparation.

What is the concentration of RNaseOUT Recombinant Ribonuclease Inhibitor in Units/microL?

RNaseOUT Recombinant Ribonuclease Inhibitor is provided at a concentration of 40 U/µL.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

What is the temperature limitation of RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019)?

RNaseOUT Recombinant Ribonuclease Inhibitor (Cat. No. 10777019) unit reaction conditions are defined at 25 degrees C. The upper temperature limit for full functionality is 40 degrees C. The enzyme half-life is decreased as temperatures increase above 40 degrees C. There is some residual activity up to 50-55 degrees C but heating at 65 degrees C will inactivate the enzyme. As RNaseOUT can be used in various applications like cDNA synthesis, RT-PCR, and in vitro transcription, the recommendation is to follow the temperature settings required for the respective method.

Find additional tips, troubleshooting help, and resources within our Nucleic Acid Purification and Analysis Support Center.

I am having trouble performing PCR amplification after DNA isolation with RNAlater-treated samples. Does RNAlater have an impact on downstream PCR applications?

RNAlater should not impact downstream PCR amplification as long as the sample has been properly cleaned before proceeding with DNA isolation, as stated in the RNAlater Stabilization Solution manual (https://assets.thermofisher.com/TFS-Assets/LSG/manuals/7020M.pdf) on page 7.

Find additional tips, troubleshooting help, and resources within our RNA Sample Collection, Protection, and Isolation Support Center.

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Certificados

N.º de loteCertificate TypeDateCatalog Number(s)
3130706Certificate of Analysis22 may 202510777019
3083251Certificate of Analysis19 feb 202510777019
3037375Certificate of Analysis15 nov 202410777019
2894759Certificate of Analysis18 jun 202410777019
2863150Certificate of Analysis10 jun 202410777019
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Citations & References (6)

Citations & References
Abstract
The catalytic domain of RNase E shows inherent 3' to 5' directionality in cleavage site selection.
Authors: Feng Yanan; Vickers Timothy A; Cohen Stanley N;
Journal:Proc Natl Acad Sci U S A
PubMed ID:12417756
'RNase E, a multifunctional endoribonuclease of Escherichia coli, attacks substrates at highly specific sites. By using synthetic oligoribonucleotides containing repeats of identical target sequences protected from cleavage by 2''-O-methylated nucleotide substitutions at specific positions, we investigated how RNase E identifies its cleavage sites. We found that the RNase E catalytic ... More
Cloning and functional characterization of HDAC11, a novel member of the human histone deacetylase family.
Authors: Gao Lin; Cueto Maria A; Asselbergs Fred; Atadja Peter;
Journal:J Biol Chem
PubMed ID:11948178
'We have cloned and characterized a human cDNA that belongs to the histone deacetylase family, which we designate as HDAC11. The predicted HDAC11 amino acid sequence reveals an open reading frame of 347 residues with a corresponding molecular mass of 39 kDa. Sequence analyses of the putative HDAC11 protein indicate ... More
Development of reverse transcription (RT)-PCR and real-time RT-PCR assays for rapid detection and quantification of viable yeasts and molds contaminating yogurts and pasteurized food products.
Authors:Bleve G, Rizzotti L, Dellaglio F, Torriani S,
Journal:Appl Environ Microbiol
PubMed ID:12839789
Reverse transcriptase PCR (RT-PCR) and real-time RT-PCR assays have been used to detect and quantify actin mRNA from yeasts and molds. Universal primers were designed based on the available fungal actin sequences, and by RT-PCR they amplified a specific 353-bp fragment from fungal species involved in food spoilage. From experiments ... More
Regulation of H-ras splice variant expression by cross talk between the p53 and nonsense-mediated mRNA decay pathways.
Authors:Barbier J, Dutertre M, Bittencourt D, Sanchez G, Gratadou L, de la Grange P, Auboeuf D,
Journal:Mol Cell Biol
PubMed ID:17709397
When cells are exposed to a genotoxic stress, a DNA surveillance pathway that involves p53 is activated, allowing DNA repair. Eukaryotic cells have also evolved a mechanism called mRNA surveillance that controls the quality of mRNAs. Indeed, mutant mRNAs carrying premature translation termination codons (PTCs) are selectively degraded by the ... More
Biochemistry of mitochondrial nitric-oxide synthase.
Authors:Elfering SL, Sarkela TM, Giulivi C
Journal:J Biol Chem
PubMed ID:12154090
We reported that the generation of nitric oxide by mitochondria is catalyzed by a constitutive, mitochondrial nitric-oxide synthase (mtNOS). Given that this production may establish the basis for a novel regulatory pathway of energy metabolism, oxygen consumption, and oxygen free radical production, it becomes imperative to identify unequivocally and characterize ... More
6 total citations

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