DMEM/F-12 KnockOut™

Q: What is the concentration of glucose in KnockOut DMEM/F-12 Medium?

KnockOut DMEM/F-12 Medium contains 3151.0 mg/L of glucose.Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center .

Medio de baja osmolalidad sin L-glutamina o tampón HEPES optimizado para el crecimiento de células madre pluripotentes inducidas o embrionariasMás información

Número de catálogo	Cantidad
12660012	500 mL

Número de catálogo 12660012

Precio (EUR)

56,25

Each

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Cantidad:

500 mL

Customize this product

Precio (EUR)

56,25

Each

Añadir al carro de la compra

Medio de baja osmolalidad sin L-glutamina o tampón HEPES optimizado para el crecimiento de células madre pluripotentes inducidas o embrionarias humanas.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Tipo de célulaCélulas madre (embrionarias), células madre (iPS, pluripotentes inducidas)

Calidad de fabricacióncGMP para dispositivos médicos, 21 CFR parte 820 e ISO 13485

Línea de productosGibco™

Tipo de productoDMEM/F-12 KnockOut

Cantidad500 mL

Condiciones de envíoTemperatura ambiente

FormularioLíquido

Con aditivosRojo de fenol

Sin aditivosSin glutamina, Sin HEPES

Unit SizeEach

Contenido y almacenamiento

Almacenar entre 2 °C y 8 °C y proteger de la luz.

Preguntas frecuentes

What is the concentration of glucose in KnockOut DMEM/F-12 Medium?

KnockOut DMEM/F-12 Medium contains 3151.0 mg/L of glucose.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Does KnockOut DMEM/F-12 contain Knockout Serum Replacement (KSR) supplement?

KnockOut DMEM/F-12 does not contain Knockout Serum Replacement (KSR) supplement; it would need to be added prior to use.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

Do you offer DMEM/F12 medium without folic acid?

We do not offer DMEM/F12 medium without folic acid as a standard catalog product. However, our Customs group can make it for you as a custom media. If you are interested, please complete this Custom order inquiry form PDF (https://www.thermofisher.com/content/dam/LifeTech/global/applied-sciences/pdfs/Bioproduction/Gibco-Custom-Media-Buffers-Request-Form.pdf) and email it to custommedia@thermofisher.com. Our Customs group will review your requirements and get back to you within a couple of days with pricing, ordering information, and lead time. After that, you have the choice of proceeding with an “official” order or declining.

Find additional tips, troubleshooting help, and resources within our Cell Culture Support Center.

How do I characterize human embryonic stem ( ES) cells?

Human ES cells are generally characterized by their typical morphology (they grow as tightly packed clusters of small cells with high ratio of nucleus to cytoplasm); surface marker expression; RT-PCR detection of stem cell-specific gene expression (such as Oct3/4, Sox2, and Nanog); alkaline phosphatase staining, and telomerase activity assay. The most commonly used ES specific surface markers include stage-specific embryonic antigens SSEA-3 and SSEA-4 for human ES cells. Other ES-specific surface antigens also include TRA-1-60 and TRA-1-81. (Science 282:1145 (1998).

How are human embryonic stem (ES) cells derived?

Human ES cells are derived from human blastocyst inner cell masses, isolated by immunosurgery with rabbit antiserum to BeWO cells (a human trophoblast cell line) (Science 282:1145 (1998)).

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